Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/42492
Title: Inflammatory signaling in dengue-infected platelets requires translation and secretion of nonstructural protein 1
Authors: Teixeira, Anna Cecillia Quirino
Rozini, Stephane Vicente
Lima, Giselle Barbosa
Coelho, Diego Rodrigues
Carneiro, Pedro Henrique
Borges, Ronaldo Mohana
Bozza, Patricia T.
Hottz, Eugenio D.
Affilliation: Universidade Federal de Juiz de Fora. Departamento de Bioquímica. Laboratório de Análise de Glicoconjugates. Laboratório de Imunotrombose. Juiz de Fora, MG, Brasil / Universidade Federal de Juiz de Fora. Departamento de Bioquímica. Laboratório de Análises de Glicoconjugates. Juiz de Fora, MG, Brasil.
Universidade Federal de Juiz de Fora. Departamento de Bioquímica. Laboratório de Análise de Glicoconjugates. Laboratório de Imunotrombose. Juiz de Fora, MG, Brasil / Universidade Federal de Juiz de Fora. Departamento de Bioquímica. Laboratório de Análises de Glicoconjugates. Juiz de Fora, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil,
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil,
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil,
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal de Juiz de Fora. Departamento de Bioquímica. Laboratório de Análise de Glicoconjugates. Laboratório de Imunotrombose. Juiz de Fora, MG, Brasil / Universidade Federal de Juiz de Fora. Departamento de Bioquímica. Laboratório de Análises de Glicoconjugates. Juiz de Fora, MG, Brasil.
Abstract: Emerging evidence identifies major contributions of platelets to inflammatory amplification in dengue, but the mechanisms of infection-driven platelet activation are not completely understood. Dengue virus nonstructural protein-1 (DENV NS1) is a viral protein secreted by infected cells with recognized roles in dengue pathogenesis, but it remains unknown whether NS1 contributes to the inflammatory phenotype of infected platelets. This study shows that recombinant DENV NS1 activated platelets toward an inflammatory phenotype that partially reproduced DENV infection. NS1 stimulation induced translocation of a-granules and release of stored factors, but not of newly synthesized interleukin-1b (IL-1b). Even though both NS1 and DENV were able to induce pro-IL-1b synthesis, only DENV infection triggered caspase-1 activation and IL-1b release by platelets. A more complete thromboinflammatory phenotype was achieved by synergistic activation of NS1 with classic platelet agonists, enhancing a-granule translocation and inducing thromboxane A2 synthesis (thrombin and platelet-activating factor), or activating caspase-1 for IL-1b processing and secretion (adenosine triphosphate). Also, platelet activation by NS1 partially depended on toll-like receptor-4 (TLR-4), but not TLR-2/6. Finally, the platelets sustained viral genome translation and replication, but did not support the release of viral progeny to the extracellular milieu, characterizing an abortive viral infection. Although DENV infection was not productive, translation of the DENV genome led to NS1 expression and release by platelets, contributing to the activation of infected platelets through an autocrine loop. These data reveal distinct, new mechanisms for platelet activation in dengue, involving DENV genome translation and NS1-induced platelet activation via platelet TLR4.
Keywords: inflammatory signaling
Platelets
Infection
Nonstructural protein 1
Dengue
keywords: Sinalização inflamatória
Plaquetas
Infecção
Dengue
Proteína não estrutural 1
Issue Date: 2020
Publisher: American Society of Hematology:
Citation: QUIRINO-TEIXEIRA, Anna Cecilia et al. Inflammatory signaling in dengue-infected platelets requires translation and secretion of nonstructural protein 1. Blood Advances, v. 4, n. 9, p. 2018-2031, May 2020.
DOI: 10.1182/bloodadvances.2019001169
ISSN: 2473-9529
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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