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FIRST GENOTYPING OF TRYPANOSOMA CRUZI FROM NATURALLY INFECTED TRIATOMA JUAZEIRENSIS, TRIATOMA MELANICA AND TRIATOMA SHERLOCKI FROM BAHIA STATE, BRAZIL
Affilliation
Universidade Federal Fluminense. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.
Universidade Federal Fluminense. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biodiversidade Entomológica. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biodiversidade Entomológica. Rio de Janeiro, RJ, Brasil
Universidade Federal Fluminense. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biodiversidade Entomológica. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biodiversidade Entomológica. Rio de Janeiro, RJ, Brasil
Abstract
Many previous studies have shown a great phylogenetic and biological
variability of Trypanosoma cruzi using different molecular and biochemical methods.
Populations of T. cruzi were initially clustered into two main lineages called TcI and
TcII by the size of the mini-exon PCR product. In the present study, 33 isolates derived
from three triatomine taxa, which belong to the Triatoma brasiliensis species complex
(Triatoma juazeirensis, Triatoma melanica and Triatoma sherlocki); collected in three
distinct areas of Bahia state were characterized by PCR. The isolates were identified
by the size of the mini-exon gene, 18S rRNA and 24S𝛼 rRNA amplicons. T. cruzi
isolates obtained in sylvatic and intradomiciliar ecotopes, derived from T. juazeirensis
and T. melanica, were identified as TcI while the parasites originated from T. sherlocki
were characterized as TcI and TcII genotypes, respectively. Those species are present
in sylvatic ecotopes but are able to infest intradomiciliar areas. Therefore, it would be
important to maintain studies in those localities of Bahia and further investigate the
possibilities of Chagas disease transmission. Human disease may occur by any T. cruzi
genotype and not only by TcII as it is the case in Amazonia.
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