Author | Damasceno, Patricia Kauanna Fonseca | |
Author | Santana, Thaís Alves de | |
Author | Santos, Girlaine Café | |
Author | Orge, Iasmim Diniz | |
Author | Silva, Daniela Nascimento | |
Author | Albuquerque, Juliana Fonseca | |
Author | Golinelli, Giulia | |
Author | Grisendi3, Giulia | |
Author | Pinelli, Massimo | |
Author | Santos, Ricardo Ribeiro dos | |
Author | Dominici, Massimo | |
Author | Soares, Milena Botelho Pereira | |
Access date | 2020-09-16T14:48:32Z | |
Available date | 2020-09-16T14:48:32Z | |
Document date | 2020 | |
Citation | DAMASCENO, Patricia Kauanna Fonseca et al. Kauanna FonsecaGenetic Engineering as a Strategy to Improve the Therapeutic Efficacy of Mesenchymal Stem/Stromal Cells in Regenerative Medicine. Frontiers in Cell and Developmental Biology, v. 8, Article 737, p. 1-24, Aug. 2020. | pt_BR |
ISSN | 2296-634X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/43400 | |
Sponsorship | Brazilian National Research Council (CNPq)/MS
grant number 443909/2018-0 and INOVA/FIOCRUZ grant
number VPPIS-001-FIO-18-70. | pt_BR |
Language | eng | pt_BR |
Publisher | Frontiers Media | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Células-tronco mesenquimais | pt_BR |
Subject in Portuguese | Engenharia genética | pt_BR |
Subject in Portuguese | Medicina regenerativa | pt_BR |
Subject in Portuguese | Terapia celular | pt_BR |
Subject in Portuguese | Terapia de genes | pt_BR |
Subject in Portuguese | Células estromais | pt_BR |
Title | Genetic Engineering as a Strategy to Improve the Therapeutic Efficacy of Mesenchymal Stem/Stromal Cells in Regenerative Medicine | pt_BR |
Type | Article | pt_BR |
DOI | 10.3389/fcell.2020.00737 | pt_BR |
Abstract | Mesenchymal stem/stromal cells (MSCs) have been widely studied in the field of regenerative medicine for applications in the treatment of several disease settings. The therapeutic potential of MSCs has been evaluated in studies in vitro and in vivo, especially based on their anti-inflammatory and pro-regenerative action, through the secretion of soluble mediators. In many cases, however, insufficient engraftment and limited beneficial effects of MSCs indicate the need of approaches to enhance their survival, migration and therapeutic potential. Genetic engineering emerges as a means to induce the expression of different proteins and soluble factors with a wide range of applications, such as growth factors, cytokines, chemokines, transcription factors, enzymes and microRNAs. Distinct strategies have been applied to induce genetic modifications with the goal to enhance the potential of MCSs. This review aims to contribute to the update of the different genetically engineered tools employed for MSCs modification, as well as the factors investigated in different fields in which genetically engineered MSCs have been tested. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Health Institute of Technology. Salvador, BA, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Health Institute of Technology. Salvador, BA, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Health Institute of Technology. Salvador, BA, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | University of Modena and Reggio Emilia. Laboratory of Cellular Therapy. Division of Oncology. Modena, Italy. | pt_BR |
Affilliation | University of Modena and Reggio Emilia. Laboratory of Cellular Therapy. Division of Oncology. Modena, Italy. | pt_BR |
Affilliation | University of Modena and Reggio Emilia. Department of Medical and Surgical Sciences for Children & Adults. Division of Plastic Surgery. Modena, Italy. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Health Institute of Technology. Salvador, BA, Brazil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Health Institute of Technology. Salvador, BA, Brazil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil. | pt_BR |
Subject | Mesenchymal stem | pt_BR |
Subject | Genetic engineering | pt_BR |
Subject | Regenerative medicine | pt_BR |
Subject | Cell therapy | pt_BR |
Subject | Gene therapy | pt_BR |
Subject | Stromal cells | pt_BR |