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HUMAN PARVOVIRUS B19 INFECTION AND HYDROPS FETALIS IN RIO DE JANEIRO, BRAZIL
Cunel, Rita C. N. et al. | Date Issued: 1996
Author
Cunel, Rita C. N.
Garcia, Aparecida G. P .
Pegado, Claudia S.
Ramos, Hilda I.
Fonseca, Maria E. F.
Clewley, Jonhatan P.
Cohen, Bernard J.
Nascimento, Jussara P.
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Centro de Ciências Médicas. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Serviço de Anatomia Patológica. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Serviço de Anatomia Patológica. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Serviço de Anatomia Patológica. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Setor de Microscopia Eletrônica. Rio de Janeiro, RJ, Brasil.
Central Public Health Laboratory. Virus Reference Division. London, UK.
Central Public Health Laboratory. Virus Reference Division. London, UK.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
Abstract
Formalin-fixed paraffin embedded lung and liver tissue from 23 cases of non immune hydrops fetalis and five control cases, in which hydrops were due to syphilis (3) and genetic causes (2), were examined for the presence of human parvovirus B19 by DNA hybridisation. Using in situ hybridisation with a biotynilated probe one positive case was detected. Using 32P-labelled probes in a dot blot assay format, five further positives were obtained. These were all confirmed as positive by a nested polymerase chain reaction assay. Electron microscopy revealed virus in all these five positive cases. The six B19 DNA positive cases of hydrops fetalis were from 1974, 1980, 1982, 1987 and 1988, four of which occurred during the second half of the year, confirming the seasonality of the disease.
Keywords in Portuguese
Parvovírus humano B19
Hydrops fetalis
Detecção de vírus
 
Keywords
Human parvovirus B19
Hydrops fetalis
Virus detection
 
Publisher
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
Citation
CUBEL, Rita C. N. et al. Human parvovirus B19 infection and hydrops fetalis in Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 2, p. 147-151, Mar./Apr. 1996.
DOI
10.1590/S0074-02761996000200003
ISSN
0074-0276