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TOWARDS THE PHYSICAL MAP OF THE TRYPANOSOMA CRUZI NUCLEAR GENOME: CONSTRUCTION OF YAC AND BAC LIBRARIES OF THE REFERENCE CLONE T. CRUZI CL-BRENER
Ferrari, I. et al. | Date Issued: 1997
Author
Ferrari, I.
Lorenzi, H.
Santos, M. R.
Brandariz, S.
Rquena, J. M.
Schijman, A.
Vázquez, M.
Silveira, J. F. da
Ben-Dov, C.
Medrano, C.
Ghio, S.
Bergami, P. López
Cano, I.
Zingalez, B.
Urmenyi, T. P.
Rondinelli, E.
González, A.
Cortes, A.
Lopez, M. C.
Thomaz, M. C.
Alonso, C.
Ramírez, J. L.
Chiurrillo, M. A.
Aldao, R. Rangel
Brandão, Adeilton
Degrave, Wim
Perrot, V.
Saumier, M.
Billaut, A.
Cohen, D.
Le Paslier, D.
Levin, M. J.
Affilliation
Escola Paulista de Medicina. São Paulo, SP, Brasil .
Escola Paulista de Medicina. São Paulo, SP, Brasil .
Escola Paulista de Medicina. São Paulo, SP, Brasil .
CBM. Madrid, España.
CBM. Madrid, España.
Escola Paulista de Medicina. São Paulo, SP, Brasil .
Escola Paulista de Medicina. São Paulo, SP, Brasil .
Escola Paulista de Medicina. São Paulo, SP, Brasil .
Escola Paulista de Medicina. São Paulo, SP, Brasil .
Escola Paulista de Medicina. São Paulo, SP, Brasil .
Escola Paulista de Medicina. São Paulo, SP, Brasil .
Escola Paulista de Medicina. São Paulo, SP, Brasil .
Escola Paulista de Medicina. São Paulo, SP, Brasil .
Universidade Federal do Rio de Janeiro. CCS. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. CCS. Rio de Janeiro, RJ, Brasil.
IPB, Granada, España
IPB. Granada, España.
IPB. Granada, España.
IPB. Granada, España.
CBM. Madrid, España.
Universidad Central de Venezuela. Caracas, Venezuela.
Universidad Central de Venezuela. Caracas, Venezuela.
USB. Sartenejas, Caracas, Venezuela.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
CEPH, 27 Rue Juliette Dodou, 75010 Paris, France.
CEPH, 27 Rue Juliette Dodou, 75010 Paris, France.
CEPH, 27 Rue Juliette Dodou, 75010 Paris, France.
CEPH, 27 Rue Juliette Dodou, 75010 Paris, France.
Sem afiliação
Sem afiliação
Abstract
Strategies to construct the physical map of the Trypanosoma cruzi nuclear genome have to capitalize on three main advantages of the parasite genome, namely (a) its small size, (b) the fact that all chromosomes can be defined, and many of them can be isolated by pulse field gel electrophoresis, and (c) the fact that simple Southern blots of electrophoretic karyotypes can be used to map sequence tagged sites and expressed sequence tags to chromosomal bands. A major drawback to cope with is the complexity of T. cruzi genetics, that hinders the construction of a comprehensive genetic map. As a first step towards physical mapping, we report the construction and partial characterization of a T. cruzi CLBrener genomic library in yeast artificial chromosomes (YACs) that consists of 2,770 individual YACs with a mean insert size of 365 kb encompassing around 10 genomic equivalents. Two libraries in bacterial artificial chromosomes (BACs) have been constructed, BACI and BACII. Both libraries represent about three genome equivalents. A third BAC library (BAC III) is being constructed. YACs and BACs are invaluable tools for physical mapping. More generally, they have to be considered as a common resource for research in Chagas disease.
Keywords in Portuguese
Trypanosoma cruzi
Projeto genoma
Mapa físico
Biblioteca YAC
Biblioteca BAC
Locais marcados por sequência (STS)
Tags de sequência expressas (EST)
 
Keywords
Trypanosoma cruzi
Genome project
Physical map
YAC library
BAC library
Sequence tagged sites (STS)
Expressed sequence tags (EST)
 
Publisher
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Citation
FERRARI, I. et al. Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 6, p. 843-852, Nov./Dec. 1997.
DOI
10.1590/S0074-02761997000600021
ISSN
0074-0206