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DETECTION OF DRUG RESISTANT MYCOBACTERIUM TUBERCULOSIS STRAINS USING KIT SIRE NITRATASE®: A MULTICENTER STUDY
Miranda, Silvana Spindola de et al. | Date Issued: 2020
Author
Miranda, Silvana Spindola de
Almeida, Isabela Neves de
Mansur, Maria de Fátima Filardi Oliveira
Figueredo, Lida Jouca de Assis
Carvalho, Wânia da Silva
Hadaad, João Paulo Amaral
Diniz, Jaciara de Lourdes do Carmo Guimarães
Groll, Andrea von
Silva, Pedro Almeida da
Lopes, Maria Luiza
Santos, Marcelo Cordeiro dos
Brito, Alexandra
Mello, Fernanda Carvalho de Queiroz
Malaquias, Thiago da Silva Santos
Croda, Julio
Pinhata, Juliana Maira Watanabe
Oliveira, Rosângela Siqueira de
Chimara, Erica
Rossetti, Maria Lúcia
Halon, Maria Laura
Lourenço, Maria Cristina
Medeiros, Reginalda Ferreira de Melo
Montes, Fátima Cristina Onofre Fandinho
Machado, Diana
Viveiros, Miguel
Kritski, Afrânio Lineu
Affilliation
Federal University of Minas Gerais. Faculty of Medicine. Mycobacteria Research Laboratory. Belo Horizonte, MG, Brazil.
Federal University of Minas Gerais. Faculty of Medicine. Mycobacteria Research Laboratory. Belo Horizonte, MG, Brazil.
Federal University of Minas Gerais. Faculty of Medicine. Mycobacteria Research Laboratory. Belo Horizonte, MG, Brazil.
Federal University of Minas Gerais. Faculty of Medicine. Mycobacteria Research Laboratory. Belo Horizonte, MG, Brazil.
Federal University of Minas Gerais. Faculty of Pharmacy. Department of Social Pharmacy. Belo Horizonte, MG, Brazil.
Federal University of Minas Gerais. Veterinary School. Department of Preventive Veterinary Medicine. Belo Horizonte, MG, Brazil.
Federal University of Rio Grande. Faculty of Medicine. Laboratory of Mycobacteria. Rio Grande, RS, Brazil.
Federal University of Rio Grande. Faculty of Medicine. Laboratory of Mycobacteria. Rio Grande, RS, Brazil.
Federal University of Rio Grande. Faculty of Medicine. Laboratory of Mycobacteria. Rio Grande, RS, Brazil.
Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Tropical Medicine Foundation Dr. Heitor Vieira Dourado. Manaus, AM, Brazil.
Tropical Medicine Foundation Dr. Heitor Vieira Dourado. Manaus, AM, Brazil.
Federal University of Rio de Janeiro. Institute of Chest Diseases. Clementino Fraga Filho University Hospital. Rio de Janeiro, RJ, Brazil.
Federal University of Rio de Janeiro. Institute of Chest Diseases. Clementino Fraga Filho University Hospital. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Fiocruz Mato Grosso do Sul. Campo Grande, MS, Brasil / Federal University of Grande Dourados. Faculty of Health Sciences. Dourados, MS, Brazil.
Adolfo Lutz Institute. Bacteriology Center. Tuberculosis and Mycobacteriosis Center. São Paulo, SP, Brazil.
Adolfo Lutz Institute. Bacteriology Center. Tuberculosis and Mycobacteriosis Center. São Paulo, SP, Brazil.
Adolfo Lutz Institute. Bacteriology Center. Tuberculosis and Mycobacteriosis Center. São Paulo, SP, Brazil.
State Secretariat of Health of Rio Grande do Sul. State Center for Health Surveillance. Center for Scientific and Technological Development. Porto Alegre, RS, Brazil.
State Secretariat of Health of Rio Grande do Sul. State Center for Health Surveillance. Center for Scientific and Technological Development. Porto Alegre, RS, Brazil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Centro de Referência Professor Hélio Fraga. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Centro de Referência Professor Hélio Fraga. Rio de Janeiro, RJ, Brasil.
NOVA University of Lisbon. Institute of Hygiene and Tropical Medicine. Medical Microbiology Unit. Global Health and Tropical Medicine. Lisboa, Portugal.
NOVA University of Lisbon. Institute of Hygiene and Tropical Medicine. Medical Microbiology Unit. Global Health and Tropical Medicine. Lisboa, Portugal.
Federal University of Rio de Janeiro. Faculty of Medicine. Tuberculosis Research Center. Rio de Janeiro, RJ, Brazil.
Abstract
(1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.
Keywords
Tuberculosis
MDR
Nitrate reductase
Technology
 
Publisher
Scielo
Citation
MIRANDA, Silvana Spindola de et al. Detection of Drug Resistant Mycobacterium Tuberculosis Strains Using Kit SIRE Nitratase®: a Multicenter Study. Brazilian Archives of Biology and Technology, v. 63, p. 1-9, 2020
DOI
10.1590/1678-4324-2020190179
ISSN
1516-8913