| Author | Alessio, Glaucia Diniz | |
| Author | Araújo, Fernanda Fortes de | |
| Author | Silva, Jéssica Spínola | |
| Author | Sales Júnior, Policarpo Ademar | |
| Author | Gomes, Matheus de Souza | |
| Author | Amaral, Laurence Rodrigues do | |
| Author | Ramírez, Juan David | |
| Author | Flórez, Carolina | |
| Author | Carvalho, Andréa Teixeira de | |
| Author | Pinheiro, Melina de Barros | |
| Author | Lana, Marta de | |
| Author | Martins Filho, Olindo Assis | |
| Access date | 2020-10-20T15:58:37Z | |
| Available date | 2020-10-20T15:58:37Z | |
| Document date | 2020 | |
| Citation | ALESSIO, Glaucia Diniz et al. Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease. Sci Rep, v. 10, 13296, 2020. https://doi.org/10.1038/s41598-020-69921-z | pt_BR |
| ISSN | 2045-2322 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/44081 | |
| Language | eng | pt_BR |
| Publisher | Nature | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Doença de chagas | pt_BR |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Title | Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1038/s41598-020-69921-z | |
| Abstract | The molecular and serological methods available for Discrete Typing Units (DTU)-specific diagnosis of Trypanosoma cruzi in chronic Chagas disease present limitations. The study evaluated the performance of Human Chagas-Flow ATE-IgG1 for universal and DTU-specific diagnosis of Chagas disease. A total of 102 sera from Chagas disease patients (CH) chronically infected with TcI, TcVI or TcII DTUs were tested for IgG1 reactivity to amastigote/(A), trypomastigote/(T) and epimastigote/(E) antigens along the titration curve (1:250–1:32,000). The results demonstrated that “AI 250/40%”, “EVI 250/30%”, “AII 250/40%”, “TII 250/40%” and “EII 250/30%” have outstanding accuracy (100%) to segregate CH from non-infected controls. The attributes “TI 4,000/50%”, “EI 2,000/50%”, “AVI 8,000/60%” and “TVI 4,000/50%” were selected for DTU-specific serotyping of Chagas disease. The isolated use of “EI 2,000/50%” provided the highest co-positivity for TcI patients (91%). The combined decision tree algorithms using the pre-defined sets of attributes showed outstanding full accuracy (92% and 97%) to discriminate “TcI vs TcVI vs TcII” and “TcI vs TcII” prototypes, respectively. The elevated performance of Human Chagas-Flow ATE-IgG1 qualifies its use for universal and TcI/TcVI/TcII-specific diagnosis of Chagas disease. These findings further support the application of this method in epidemiological surveys, post-therapeutic monitoring and clinical outcome follow-ups for Chagas disease. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil/ Universidade Federal de São João Del-Rei. Histopatologia e Análises Clínicas. Laboratório CT-Infra II. Divinópolis, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de São João Del-Rei. Histopatologia e Análises Clínicas. Laboratório CT-Infra II. Divinópolis, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Genômica Funcional e Proteômica de Leishmania spp e Trypanosoma cruzi. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, Brazil | pt_BR |
| Affilliation | Universidad del Rosario. Facultad de Ciencias Naturales. Departamento de Biología. Grupo de Investigaciones Microbiológicas-UR. Bogotá, Colombia | pt_BR |
| Affilliation | Instituto Nacional de Salud. Bogotá, Colombia | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de São João Del-Rei. Histopatologia e Análises Clínicas. Laboratório CT-Infra II. Divinópolis, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil/ Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Doença de Chagas Ouro Preto, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | Chagas disease | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
