Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/44551
Title: Vertical stratification of sand fly diversity in relation to natural infections of Leishmania sp. and blood‑meal sources in Jamari National Forest, Rondônia State, Brazil
Authors: Leão, Paula de Oliveira
Júnior, Antonio Marques Pereira
Paulo, Paula Frassinetti Medeiros de
Carvalho, Luis Paulo Costa
Souza, Ana Beatriz Nascimento
Silva, Michelli Santos da
Castro, Thaís Santos
Freitas, Moisés Thiago de Souza
Rodrigues, Moreno Magalhães de Souza
Ferreira, Gabriel Eduardo Melim
Medeiros, Jansen Fernandes
Affilliation: Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de Pós Graduação em Biologia Experimental. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil / Instituto Nacional de Ciência e Tecnologia de Epidemiologia da Amazônia Ocidental-INCT-EpiAmO. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de Pós Graduação em Biologia Experimental. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de Pós Graduação em Biologia Experimental. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil.
Universidade Federal de Pernambuco. Departamento de Genética. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil / Instituto Nacional de Ciência e Tecnologia de Epidemiologia da Amazônia Ocidental-INCT-EpiAmO. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil / Instituto Nacional de Ciência e Tecnologia de Epidemiologia da Amazônia Ocidental-INCT-EpiAmO. Porto Velho, RO, Brasil.
Abstract: Background: Almost 1000 cases of American cutaneous leishmaniasis have been registered yearly in Rondônia State, Brazil. Little is known about the Leishmania transmission cycle (vectors and reservoirs) in the state. This study aimed to evaluate sand fly fauna from two vertical stratification layers in order to identify potential vectors and their blood-meal sources. Methods: The study was conducted in Jamari National Forest. Sand flies were collected in the canopy (15 m) and at ground level (1 m) using HP light traps during four months, February, April, August and October, 2018. Insects were identified to the species level, and females were subjected to DNA extraction and PCR targeting minicircle kDNA and hsp70 (for Leishmania detection and species identification), and cytb (to identify blood-meal sources). Exploratory data analysis was used to determine mean of abundance and species richness between stratifications. The hsp70 and cytb sequences were analyzed and compared with sequences from GenBank. Results: Overall, 68 species were identified from 15,457 individuals. On the Potosi trail, 7531 individuals of 49 species were collected; canopy captures totaled 6463 individuals of 46 species, while ground captures totaled 1068 individuals of 38 species. On the Santa Maria trail, 7926 individuals of 61 species were collected; canopy captures totaled 6136 individuals of 51 species, while ground captures totaled 1790 individuals of 53 species. A total of 23 pools were positive for kDNA (canopy n = 21, ground n = 2). Only two samples were sequenced for hsp70 (both in canopy); one sequence exhibited similarity with Leishmania braziliensis (Lutzomyia davisi pool) and another with L. naiffi (Lu. antunesi pool). The cytb fragment was amplified in 11 of 86 samples. Sample sequencing identified cytb DNA from 5 bloodmeal sources: Micrastur gilvicollis, Psophia viridis, Tamandua tetradactyla, Homo sapiens and Choloepus didactylus. Conclusions: Sand fly fauna is more diverse in the canopy than at ground level. Factors such as blood-meal sources, resting sites, and abiotic components probably contribute to high abundance in the canopy. Our results reinforce the possibility that Lu. antunesi and Lu. davisi participate in Leishmania transmission in forest environments and may play an important role in transmission from sylvatic to human hosts.
Keywords: Zoonotic disease
Leishmaniasis
Vectors
Stratification
Canopy
Ground
Issue Date: 2020
Publisher: Parasites & Vectors
Citation: LEÃO, Paula de Oliveira et al. Vertical stratification of sand fly diversity in relation to natural infections of Leishmania sp. and blood‑meal sources in Jamari National Forest, Rondônia State, Brazil. Parasites Vectors, p. 1-13, 2020.
DOI: 10.1186/s13071-020-04295-9
ISSN: 1756-3305
Copyright: open access
Appears in Collections:RO - Artigos de Periódicos
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