Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/44562
Title: Prevalence and genetic diversity of astroviruses in children with and without diarrhea in São Luís, Maranhão, Brazil
Authors: Gabbay, Yvone B.
Luz, Cláudia Regina N. E. da
Costa, Ivete V.
Pepino, Elielma L. Cavalcante
Sousa, Maisa S.
Oliveira, Krynssya K.
Wanzeller, Ana Lucia Monteiro
Mascarenhas, Joana D'Arc P.
Leite, José Paulo G.
Linhares, Alexandre C.
Affilliation: Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Seção de Virologia. Ananindeua, PA, Brasil.
Hospital Universitário Materno Infantil. São Luís, MA, Brasil.
Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Seção de Virologia. Ananindeua, PA, Brasil.
Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Seção de Virologia. Ananindeua, PA, Brasil.
Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Seção de Virologia. Ananindeua, PA, Brasil.
Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Seção de Virologia. Ananindeua, PA, Brasil.
Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Seção de Virologia. Ananindeua, PA, Brasil.
Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Seção de Virologia. Ananindeua, PA, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.
Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Seção de Virologia. Ananindeua, PA, Brasil.
Abstract: Human astroviruses (HAstV) have been increasingly identified as important etiological agents of acute gastroenteritis in children up to five years old. The aim of this study was to determine the prevalence and genotype diversity of HAstV in children with symptomatic and asymptomatic infections in São Luís, Maranhão, Brazil. From June 1997 to July 1999 a total of 183 fecal samples 84 from symptomatic and 99 from asymptomatic children were tested by enzyme immunoassay for HAstV. Prevalence rates were found to be 11 and 3% for symptomatic and asymptomatic children, respectively. Reverse transcription-polymerase chain reaction (RT-PCR) was carried out in 46 specimens (26 symptomatic and 20 asymptomatic) including the 12 samples that were positive by enzyme immunoassay (EIA). The overall positivity yielded by both methods was 8% (15/184); of these, 11% (9/84) for symptomatic and 5% (5/99) for those without symptoms or signs. Sequence analysis of amplicons revealed that HAstV-1 genotype was the most prevalent, accounting for 60% of isolates. Genotypes 2, 3, 4, and 5 were also detected, as one single isolate (10%) for each type. Variations in the sequences were observed when Brazilian isolates were compared to prototype strains identified in the United Kingdom. No seasonal pattern of occurrence was observed during these two years of study, and peak detection rate was observed in children aged between 3 and 6 months in the symptomatic group, and between 18 and 24 months in the controls.
Keywords: Human astroviruses (HAstV)
Diarrhea
Children
São Luís, Maranhão
Brazil
keywords: Mamastrovirus
Diarreia
Crianças
Maranhão
Brasil
DeCS: Mamastrovirus
Issue Date: 2005
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
Citation: GABBAY, Yvone B. et al. Prevalence and genetic diversity of astroviruses in children with and without diarrhea in São Luís, Maranhão, Brazil, Memórias do Instituto Oswaldo Cruz, Rio deJaneiro, v. 100, n. 7, p. 709-714, Nov. 2005.
DOI: 10.1590/S0074-02762005000700004
ISSN: 0074-0276
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos
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