| Author | Sequeira, Patrícia Carvalho de | |
| Author | Fonseca, Leila de Souza | |
| Author | Silva, Marlei Gomes da | |
| Author | Saad, Maria Helena Féres | |
| Access date | 2020-11-20T20:04:14Z | |
| Available date | 2020-11-20T20:04:14Z | |
| Document date | 2005 | |
| Citation | SEQUEIRA. Patrícia Carvalho de et al. Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 7, p. 743-748, Nov. 2005. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/44564 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Mycobacterium avium | pt_BR |
| Subject in Portuguese | AIDS | pt_BR |
| Subject in Portuguese | Micobactérias não Tuberculosas | pt_BR |
| Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
| Subject in Portuguese | Polimorfismo de Fragmento de Restrição | pt_BR |
| Title | Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02762005000700012 | |
| Abstract | Simple double repetitive element polymerase chain reaction (MaDRE-PCR) and Pvu II-IS1245 restriction fragment length polymorphism (RFLP) typing methods were used to type 41 Mycobacterium avium isolates obtained
from 14 AIDS inpatients and 10 environment and animals specimens identified among 53 mycobacteria isolated
from 237 food, chicken, and pig. All environmental and animals strains showed orphan patterns by both methods. By
MaDRE-PCR four patients, with multiple isolates, showed different patterns, suggesting polyclonal infection that
was confirmed by RFLP in two of them. This first evaluation of MaDRE-PCR on Brazilian M. avium strains demonstrated that the method seems to be useful as simple and less expensive typing method for screening genetic diversity
in M. avium strains on selected epidemiological studies, although with limitation on analysis identical patterns
except for one band. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | IS1254-restriction fragment length polymorphism | pt_BR |
| Subject | Mycobacterium avium | pt_BR |
| Subject | MaDRE-polymerase chain reaction | pt_BR |
| Subject | Nontuberculous mycobacteria | pt_BR |
| Subject | AIDS | pt_BR |
| DeCS | Micobactérias não Tuberculosas | pt_BR |
| DeCS | Células-Tronco | pt_BR |
| DeCS | Polimorfismo de Fragmento de Restrição | pt_BR |
| e-ISSN | 1678-8060 | |
