Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/44564
Title: Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains
Authors: Sequeira, Patrícia Carvalho de
Fonseca, Leila de Souza
Silva, Marlei Gomes da
Saad, Maria Helena Féres
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Rio de Janeiro, RJ, Brasil.
Abstract: Simple double repetitive element polymerase chain reaction (MaDRE-PCR) and Pvu II-IS1245 restriction fragment length polymorphism (RFLP) typing methods were used to type 41 Mycobacterium avium isolates obtained from 14 AIDS inpatients and 10 environment and animals specimens identified among 53 mycobacteria isolated from 237 food, chicken, and pig. All environmental and animals strains showed orphan patterns by both methods. By MaDRE-PCR four patients, with multiple isolates, showed different patterns, suggesting polyclonal infection that was confirmed by RFLP in two of them. This first evaluation of MaDRE-PCR on Brazilian M. avium strains demonstrated that the method seems to be useful as simple and less expensive typing method for screening genetic diversity in M. avium strains on selected epidemiological studies, although with limitation on analysis identical patterns except for one band.
Keywords: IS1254-restriction fragment length polymorphism
Mycobacterium avium
MaDRE-polymerase chain reaction
Nontuberculous mycobacteria
AIDS
keywords: Mycobacterium avium
AIDS
Micobactérias não Tuberculosas
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
DeCS: Micobactérias não Tuberculosas
Células-Tronco
Polimorfismo de Fragmento de Restrição
Issue Date: 2005
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
Citation: SEQUEIRA. Patrícia Carvalho de et al. Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 7, p. 743-748, Nov. 2005.
DOI: 10.1590/S0074-02762005000700012
ISSN: 0074-0276
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos
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