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MULTI-OMIC ANALYSES OF PLASMA CYTOKINES, LIPIDOMICS, AND TRANSCRIPTOMICS DISTINGUISH TREATMENT OUTCOMES IN CUTANEOUS LEISHMANIASIS
Santos, Hayna Malta et al. | Date Issued: 2020
Author
Santos, Hayna Malta
Fukutani, Kyoshi F.
Sorgi, Carlos A.
Queiroz, Artur T. L.
Nardini, Viviane
Silva, Juliana
Lago, Alex
Carvalho, Lucas P.
Machado, Paulo L. R.
Bozza, Patrícia T.
Costa, Jaqueline França
Faccioli, Lúcia Helena
Carvalho, Edgar M.
Andrade, Bruno de Bezerril
Borges, Viviane M.
Affilliation
Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Muniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Muniz. Saçvador, BA, Brasil / Multinational Organization Network Sponsoring Translational and Epidemiological Research. Salvador, BA, Brazil.
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Ribeirão Preto, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Muniz. Salvador, BA, Brasil / Multinational Organization Network Sponsoring Translational and Epidemiological Research. Salvador, BA, Brazil.
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Ribeirão Preto, SP, Brasil.
Universidade Federal da Bahia. Serviço de Imunologia, C-HUPES. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Muniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Serviço de Imunologia, C-HUPES. Salvador, BA, Brasil.
Universidade Federal da Bahia. Serviço de Imunologia, C-HUPES. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Muniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Serviço de Imunologia, C-HUPES. Salvador, BA, Brasil.
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Ribeirão Preto, SP, Brasil.
Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Muniz. Salvador, BA, Brasil.


Abstract
Leishmania braziliensis infection frequently results in cutaneous leishmaniasis (CL). An increase in incidence of drug-resistant CL leading to treatment failure has been reported. Identification of reliable predictors of treatment outcomes is necessary to optimize patient care. Here, we performed a prospective case-control study in which plasma levels of cytokines and lipid mediators were assessed at different time points during antileishmanial therapy in patients with CL from Brazil. Multidimensional analyses were employed to describe a combination of biomarkers able to predict and characterize treatment failure. We found a biosignature influenced mainly by plasma levels of lipid mediators that accurately predicted treatment failure. Furthermore, transcriptomic analysis of a publicly available data set revealed that expression levels of genes related to lipid metabolism measured in skin lesions could distinguish treatment outcomes in CL. Thus, activation of pathways linked to lipid biosynthesis predicts treatment failure in CL. The biomarkers identified may be further explored as therapeutic targets.
Keywords in Portuguese
Análises multiômicas
Citocinas plasmáticas
Lipidômica
Transcriptômica
Resultados no tratamento
 
Keywords
Cutaneous Leishmaniasis
Multi-omic analysis
Plasma Cytokines
Transcriptomics
Treatment
 
Publisher
Science
Citation
SANTOS, Hayna Malta et al. Multi-omic Analyses of Plasma Cytokines, Lipidomics, and Transcriptomics Distinguish Treatment Outcomes in Cutaneous Leishmaniasis. iScience, v. 23, n. 12, 101840, 30p, Dec. 2020.
DOI
10.1016/j.isci.2020.101840