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GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF ENTEROTOXIGENIC ESCHERICHIA COLI (ETEC) STRAINS ISOLATED IN RIO DE JANEIRO CITY, BRAZIL
Molecular characterization
ETEC serotyping
Cell adherence
Colonization factor antigen
Author
Affilliation
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Departamento de Ciências Biológicas. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Departamento de Microbiologia Médica. Rio de Janeiro, RJ, Brazil.
Universidade Federal de São Paulo. Escola Paulista de Medicina. Disciplina de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.
Universidade do Estado do Rio de Janeiro. d Disciplina de Microbiologia e Imunologia. Rio de Janeiro, RJ, Brazil.
Instituto Adolfo Lutz. Seção de Bacteriologia. São Paulo, SP, Brazil.
Universidade Federal do Rio de Janeiro. Laboratório de Fisiologia Celular. Rio de Janeiro, RJ, Brazil.
Universidade Federal do Rio de Janeiro. Laboratório de Fisiologia Celular. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brazil.
Universidade Federal do Rio de Janeiro. Departamento de Microbiologia Médica. Rio de Janeiro, RJ, Brazil.
Universidade Federal de São Paulo. Escola Paulista de Medicina. Disciplina de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.
Universidade do Estado do Rio de Janeiro. d Disciplina de Microbiologia e Imunologia. Rio de Janeiro, RJ, Brazil.
Instituto Adolfo Lutz. Seção de Bacteriologia. São Paulo, SP, Brazil.
Universidade Federal do Rio de Janeiro. Laboratório de Fisiologia Celular. Rio de Janeiro, RJ, Brazil.
Universidade Federal do Rio de Janeiro. Laboratório de Fisiologia Celular. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brazil.
Universidade Federal do Rio de Janeiro. Departamento de Microbiologia Médica. Rio de Janeiro, RJ, Brazil.
Abstract
Enterotoxigenic Escherichia coli (ETEC) strains have been implicated as important etiological agents of diarrheal disease, especially in developing countries. This group of microorganisms has been associated with a diverse range of genotypic and phenotypic markers. In the present study, 21 ETEC isolates previously defined according to the toxigenic genotypes, were characterized on the basis of O:H typing, cell adherence patterns, and colonization factors (CFs) antigens. Genetic diversity was investigated by random amplification polymorphic DNA (RAPD-PCR), pulsed-field gel electrophoresis (PFGE) and multilocus enzyme electrophoresis (MLEE). LT-I probe-positive isolates belonged to serotypes ONT:HNT, O7:H24, O48:H21, O88:H25, O148:H28, O159:H17 and O159:H21. ST-h probe-positive isolates belonged to serotypes O159:H17, O148:H28 and O6:H-. Serotypes O148:H28, 159:H17 and O6:H- were associated with the CS6, CFA/ I and CS1 CS3 antigens, respectively. Most ETEC strains exhibited a diffuse pattern of adherence to cultured epithelial cells. In general, phenotypic and genotypic characteristics correlated well. RAPD-PCR, PFGE and MLEE showed reproducibility and good discriminatory potential. The application of molecular typing systems allowed the detection of significant diversity among the isolates, indicating a nonclonal origin and revealing intra-serotype variation overlooked by classical epidemiological approaches. The phenotypic and genotypic diversity observed lead us to recommend the use of different typing systems in order to elucidate the epidemiology of ETEC infection. D 2003 Federation of uropean Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Keywords
Enterotoxigenic Escherichia coliMolecular characterization
ETEC serotyping
Cell adherence
Colonization factor antigen
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