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RECOMBINANT PLASMODIUM VIVAX CIRCUMSPOROZOITE SURFACE PROTEIN ALLELIC VARIANTS: ANTIBODY RECOGNITION BY INDIVIDUALS FROM THREE COMMUNITIES IN THE BRAZILIAN AMAZON
Soares, Isabel Ferreira et al. | Date Issued: 2020
Author
Soares, Isabel Ferreira
Camacho, César López
Silva, Rodrigo Nunes Rodrigues da
Matos, Ada da Silva
Baptista, Barbara de Oliveira
Totino, Paulo Renato Rivas
Souza, Rodrigo Medeiros de
Harrison, Kate
Gimenez, Alba Marina
Freitas, Elisângela Oliveira de
Kim, Young Chan
Ferreira, Joseli Oliveira
Daniel‑Ribeiro, Cláudio Tadeu
Reyes‑Sandova, Arturo
Pratt‑Riccio, Lilian Rose
Lima Junior, Josué da Costa
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.
Nuffield Department of Medicine, The Jenner Institute, The Henry Wellcome Building for Molecular Physiology. University of Oxford, Oxford, UK.
Fundação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Laboratório de Tecnologia em Anticorpos Monoclonais. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Acre. Centro de Pesquisa em Doenças Infecciosas. Centro Multidisciplinar, Campus Floresta. Rio Branco, AC, Brasil.
Nuffield Department of Medicine, The Jenner Institute, The Henry Wellcome Building for Molecular Physiology. University of Oxford, Oxford, UK.
Nuffield Department of Medicine, The Jenner Institute, The Henry Wellcome Building for Molecular Physiology. University of Oxford, Oxford, UK.
Nuffield Department of Medicine, The Jenner Institute, The Henry Wellcome Building for Molecular Physiology. University of Oxford, Oxford, UK.
Nuffield Department of Medicine, The Jenner Institute, The Henry Wellcome Building for Molecular Physiology. University of Oxford, Oxford, UK.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Centro de Pesquisa, Diagnóstico e Tratamento em Malária. Rio de Janeiro, RJ, Brasil.
Nuffield Department of Medicine, The Jenner Institute, The Henry Wellcome Building for Molecular Physiology. University of Oxford, Oxford, UK.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.
Abstract
Circumsporozoite protein (CSP) variants of P. vivax, besides having variations in the protein repetitive portion, can differ from each other in aspects such as geographical distribution, intensity of transmission, vectorial competence and immune response. Such aspects must be considered to P. vivax vaccine development. Therefore, we evaluated the immunogenicity of novel recombinant proteins corresponding to each of the three P. vivax allelic variants (VK210, VK247 and P. vivax-like) and of the C-terminal region (shared by all PvCSP variants) in naturally malaria-exposed populations of Brazilian Amazon. Our results demonstrated that PvCSP-VK210 was the major target of humoral immune response in studied population, presenting higher frequency and magnitude of IgG response. The IgG subclass profile showed a prevalence of cytophilic antibodies (IgG1 and IgG3), that seem to have an essential role in protective immune response. Differently of PvCSP allelic variants, antibodies elicited against C-terminal region of protein did not correlate with epidemiological parameters, bringing additional evidence that humoral response against this protein region is not essential to protective immunity. Taken together, these findings increase the knowledge on serological response to distinct PvCSP allelic variants and may contribute to the development of a global and effective P. vivax vaccine.
Keywords in Portuguese
Plasmodium Recombinante
Superfície circunsporozoíta vivax
Proteína alélica
Anticorpo
Amazônia brasileira
 
Keywords
Recombinant Plasmodium
Vivax circumsporozoite surface
Protein allelic
Antibody
Brazilian Amazon
 
Publisher
Nature Research
Citation
SOARES, Isabel Ferreira et al. Recombinant Plasmodium vivax circumsporozoite surface protein allelic variants: antibody recognition by individuals from three communities in the Brazilian Amazon. Scientifc Reports, v. 10, 14020, 14p, 2020.
DOI
/10.1038/s41598-020-70893-3