| Author | Angel-Camilo, Karen Leonor | |
| Author | Guerrero-Vargas, Jimmy Alexander | |
| Author | Carvalho, Emanuella Feitosa de | |
| Author | Silva, Karine Lima | |
| Author | Siqueira, Rodrigo José Bezerra de | |
| Author | Freitas, Lyara Barbosa Nogueira | |
| Author | Sousa, João Antônio Costa de | |
| Author | Mota, Mario Rogério Lima | |
| Author | Santos, Armênio Aguiar dos | |
| Author | Ferreira, Ana Gisele da Costa Neves | |
| Author | Havt, Alexandre | |
| Author | Leal, Luzia Kalyne Almeida Moreira | |
| Author | Magalhães, Pedro Jorge Caldas | |
| Access date | 2021-01-12T18:48:18Z | |
| Available date | 2021-01-12T18:48:18Z | |
| Document date | 2020 | |
| Citation | ANGEL-CAMILO, Karen Leonor et al. Disorders on cardiovascular parameters in rats and in human blood cells caused by Lachesis acrochorda snake venom. Toxicon, v. 184, p. 180-191, June 2020. | pt_BR |
| ISSN | 0041-0101 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/45656 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Hipotensão | pt_BR |
| Subject in Portuguese | Veneno de cobra | pt_BR |
| Subject in Portuguese | Agregação de plaquetas | pt_BR |
| Subject in Portuguese | Vasodilatação | pt_BR |
| Subject in Portuguese | Lachesis | pt_BR |
| Title | Disorders on cardiovascular parameters in rats and in human blood cells caused by Lachesis acrochorda snake venom | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.toxicon.2020.06.009 | |
| Abstract | In Colombia, Lachesis acrochorda causes 2–3% of all snake envenomations. The accidents promote a high mortality
rate (90%) due to blood and cardiovascular complications. Here, the effects of the snake venom of
L. acrochorda (SVLa) were analyzed on human blood cells and on cardiovascular parameters of rats. SVLa
induced blood coagulation, as measured by the prothrombin time test, but did not reduce the cell viability of
neutrophils and platelets evaluated by the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT)
reduction assay and by the lactate dehydrogenase (LDH) enzyme assay. In fact, SVLa increased the absorbance in
tests made with platelets subjected to the MTT assay. SVLa induced platelet aggregation whose magnitude was
comparable to that of the positive control adenosine diphosphate (ADP), and occurred earlier with increasing
SVLa concentration. Acetylsalicylic acid (ASA, a cyclooxygenase inhibitor) or clopidogrel (an ADP receptor
blocker) inhibited the aggregating effect of SVLa. Inhibition of SVLa-elicited platelet aggregation also resulted
from the treatment with disodium ethylenediaminetetraacetate (Na2-EDTA; metalloproteinase inhibitor) and
with 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF, serine protease inhibitor). In isolated
right atrium of rats, SVLa increased slightly, but significantly, the magnitude of the spontaneous contractions
and, in isolated rat aorta, SVLa relaxed KCl- or phenylephrine-induced contractions. In vivo, SVLa induced hypotension
and bradycardia in rats, with detection of hemorrhage in pulmonary and renal tissues. Altogether,
under experimental conditions, SVLa induced blood coagulation, platelet aggregation, hypotension and bradycardia.
Part of the effects presented here may be explained by the presence of snake venom metalloproteinases
(SVMPs) and snake venom serine proteases (SVSPs), constituents of SVLa. | pt_BR |
| Affilliation | Universidade Federal Do Ceará. Faculdade de Medicina. Departamento de Fisiologia e Farmacologia, Fortaleza, CE, Brasil / Universidad del Cauca. Centro de Investigaciones Biommédicas-Bioterio, Grupo de Investigaciones Herpetológicas y Toxinológicas. Departamento de Biología. Facultad de Ciencias Naturales, Rxactas y de la Educación. Popayán. Colombia. | pt_BR |
| Affilliation | Universidad del Cauca. Centro de Investigaciones Biommédicas-Bioterio, Grupo de Investigaciones Herpetológicas y Toxinológicas. Departamento de Biología. Facultad de Ciencias Naturales, Rxactas y de la Educación. Popayán. Colombia. | pt_BR |
| Affilliation | Universidade Federal Do Ceará. Faculdade de Medicina. Departamento de Fisiologia e Farmacologia, Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Universidade Federal Do Ceará. Faculdade de Medicina. Departamento de Fisiologia e Farmacologia, Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Universidade Federal Do Ceará. Faculdade de Medicina. Departamento de Fisiologia e Farmacologia, Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Ceará. Faculdade de Farmácia, Odontologia e Enfermagem. Departamento de Farmácia. Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Ceará. Faculdade de Farmácia, Odontologia e Enfermagem. Departamento de Farmácia. Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Universidade Federal Do Ceará. Faculdade de Medicina. Departamento de Fisiologia e Farmacologia, Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Universidade Federal Do Ceará. Faculdade de Medicina. Departamento de Fisiologia e Farmacologia, Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Do Ceará. Faculdade de Medicina. Departamento de Fisiologia e Farmacologia, Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Ceará. Faculdade de Farmácia, Odontologia e Enfermagem. Departamento de Farmácia. Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Universidade Federal Do Ceará. Faculdade de Medicina. Departamento de Fisiologia e Farmacologia, Fortaleza, CE, Brasil. | pt_BR |
| Subject | Snake venom | pt_BR |
| Subject | Hypotension | pt_BR |
| Subject | Platelet Aggregation | pt_BR |
| Subject | Lachesis | pt_BR |
| Subject | Vasodilation | pt_BR |
