Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/45656
Title: Disorders on cardiovascular parameters in rats and in human blood cells caused by Lachesis acrochorda snake venom
Authors: Angel-Camilo, Karen Leonor
Guerrero-Vargas, Jimmy Alexander
Carvalho, Emanuella Feitosa de
Silva, Karine Lima
Siqueira, Rodrigo José Bezerra de
Freitas, Lyara Barbosa Nogueira
Sousa, João Antônio Costa de
Mota, Mario Rogério Lima
Santos, Armênio Aguiar dos
Ferreira, Ana Gisele da Costa Neves
Havt, Alexandre
Leal, Luzia Kalyne Almeida Moreira
Magalhães, Pedro Jorge Caldas
Affilliation: Universidade Federal Do Ceará. Faculdade de Medicina. Departamento de Fisiologia e Farmacologia, Fortaleza, CE, Brasil / Universidad del Cauca. Centro de Investigaciones Biommédicas-Bioterio, Grupo de Investigaciones Herpetológicas y Toxinológicas. Departamento de Biología. Facultad de Ciencias Naturales, Rxactas y de la Educación. Popayán. Colombia.
Universidad del Cauca. Centro de Investigaciones Biommédicas-Bioterio, Grupo de Investigaciones Herpetológicas y Toxinológicas. Departamento de Biología. Facultad de Ciencias Naturales, Rxactas y de la Educación. Popayán. Colombia.
Universidade Federal Do Ceará. Faculdade de Medicina. Departamento de Fisiologia e Farmacologia, Fortaleza, CE, Brasil.
Universidade Federal Do Ceará. Faculdade de Medicina. Departamento de Fisiologia e Farmacologia, Fortaleza, CE, Brasil.
Universidade Federal Do Ceará. Faculdade de Medicina. Departamento de Fisiologia e Farmacologia, Fortaleza, CE, Brasil.
Universidade Federal do Ceará. Faculdade de Farmácia, Odontologia e Enfermagem. Departamento de Farmácia. Fortaleza, CE, Brasil.
Universidade Federal do Ceará. Faculdade de Farmácia, Odontologia e Enfermagem. Departamento de Farmácia. Fortaleza, CE, Brasil.
Universidade Federal Do Ceará. Faculdade de Medicina. Departamento de Fisiologia e Farmacologia, Fortaleza, CE, Brasil.
Universidade Federal Do Ceará. Faculdade de Medicina. Departamento de Fisiologia e Farmacologia, Fortaleza, CE, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Universidade Federal Do Ceará. Faculdade de Medicina. Departamento de Fisiologia e Farmacologia, Fortaleza, CE, Brasil.
Universidade Federal do Ceará. Faculdade de Farmácia, Odontologia e Enfermagem. Departamento de Farmácia. Fortaleza, CE, Brasil.
Universidade Federal Do Ceará. Faculdade de Medicina. Departamento de Fisiologia e Farmacologia, Fortaleza, CE, Brasil.
Abstract: In Colombia, Lachesis acrochorda causes 2–3% of all snake envenomations. The accidents promote a high mortality rate (90%) due to blood and cardiovascular complications. Here, the effects of the snake venom of L. acrochorda (SVLa) were analyzed on human blood cells and on cardiovascular parameters of rats. SVLa induced blood coagulation, as measured by the prothrombin time test, but did not reduce the cell viability of neutrophils and platelets evaluated by the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay and by the lactate dehydrogenase (LDH) enzyme assay. In fact, SVLa increased the absorbance in tests made with platelets subjected to the MTT assay. SVLa induced platelet aggregation whose magnitude was comparable to that of the positive control adenosine diphosphate (ADP), and occurred earlier with increasing SVLa concentration. Acetylsalicylic acid (ASA, a cyclooxygenase inhibitor) or clopidogrel (an ADP receptor blocker) inhibited the aggregating effect of SVLa. Inhibition of SVLa-elicited platelet aggregation also resulted from the treatment with disodium ethylenediaminetetraacetate (Na2-EDTA; metalloproteinase inhibitor) and with 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF, serine protease inhibitor). In isolated right atrium of rats, SVLa increased slightly, but significantly, the magnitude of the spontaneous contractions and, in isolated rat aorta, SVLa relaxed KCl- or phenylephrine-induced contractions. In vivo, SVLa induced hypotension and bradycardia in rats, with detection of hemorrhage in pulmonary and renal tissues. Altogether, under experimental conditions, SVLa induced blood coagulation, platelet aggregation, hypotension and bradycardia. Part of the effects presented here may be explained by the presence of snake venom metalloproteinases (SVMPs) and snake venom serine proteases (SVSPs), constituents of SVLa.
Keywords: Snake venom
Hypotension
Platelet Aggregation
Lachesis
Vasodilation
keywords: Hipotensão
Veneno de cobra
Agregação de plaquetas
Vasodilatação
Lachesis
Issue Date: 2020
Publisher: Elsevier
Citation: ANGEL-CAMILO, Karen Leonor et al. Disorders on cardiovascular parameters in rats and in human blood cells caused by Lachesis acrochorda snake venom. Toxicon, v. 184, p. 180-191, June 2020.
DOI: 10.1016/j.toxicon.2020.06.009
ISSN: 0041-0101
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos
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