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FIRST REPORT OF HUMAN ASTROVIRUS MLB2 IN BRAZIL DETECTED IN FECES OF CHILDREN WITH ACUTE GASTROENTERITIS LIVING IN THE STATE OF RORAIMA, NORTHERN BRAZIL
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tHuman astrovirus (HAstV) 1–8 and highly divergent HAstVMLB1−3 genotypes have beendetected in children both with and without acute gastroenteritis (AGE). One hundred andseventy fecal samples from children (≤5 years old) living in the Amazon region were eval-uated for the presence of HAstV1–8, HAstV MLB1−3 and HAstVVA1−3, using an usualRT-PCR protocol and a new protocol with specific primers designed to detect HAstVMLB1−3.HAstVMLB1 and HAstV MLB2, as well as the HAstV3 and 5 genotypes were detected.HAstVMLB1−2 genotype was detected for the first time in Brazil at a frequency of 3.5%(6/170).
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