| Author | Sarmento, Sylvia Kahwage | |
| Author | Guerra, Caroline Rezende | |
| Author | Malta, Fábio Correia | |
| Author | Coutinho, Ricardo | |
| Author | Miagostovich, Marize Pereira | |
| Author | Fumian, Tulio Machado | |
| Access date | 2021-02-21T13:33:04Z | |
| Available date | 2021-02-21T13:33:04Z | |
| Document date | 2020 | |
| Citation | SARMENTO, Sylvia Kahwage et al. Human norovirus detection in bivalve shellfish in Brazil and evaluation of viral infectivity using PMA treatment. Marine Pollution Bulletin, v. 157, p. 1-9, June 2020. | pt_BR |
| ISSN | 0025-326X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/46121 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Norovirus | pt_BR |
| Subject in Portuguese | Ostras | pt_BR |
| Subject in Portuguese | Mexilhões | pt_BR |
| Subject in Portuguese | Tratamento PMA | pt_BR |
| Subject in Portuguese | Reserva marinha | pt_BR |
| Subject in Portuguese | Rio de Janeiro | pt_BR |
| Title | Human norovirus detection in bivalve shellfish in Brazil and evaluation of viral infectivity using PMA treatment | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.marpolbul.2020.111315 | |
| Abstract | Noroviruses are the most common cause of gastroenteritis outbreaks in humans and bivalve shellfish consumption
is a recognized route of infection. Our aim was to detect and characterize norovirus in bivalves from a
coastal city of Brazil. Nucleic acid was extracted from the bivalve's digestive tissue concentrates using magnetic
beads. From March 2018 to June 2019, 77 samples were screened using quantitative RT-PCR. Noroviruses were
detected in 41.5%, with the GII being the most prevalent (37.7%). The highest viral load was 3.5 × 106 and
2.5 × 105 GC/g in oysters and mussels, respectively. PMA-treatment demonstrated that a large fraction of the
detected norovirus corresponded to non-infectious particles. Genetic characterization showed the circulation of
the GII.2[P16] and GII.4[P4] genotypes. Norovirus detection in bivalves reflects the anthropogenic impact on
marine environment and serves as an early warning for the food-borne disease outbreaks resulting from the
consumption of contaminated molluscs. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vorologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto de Estudos do Mar Almirante Paulo Moreira. Departamento de Biotecnologia Marinha. Laboratório de Genética Marinha. Arraial do Cabo, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vorologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto de Estudos do Mar Almirante Paulo Moreira. Departamento de Biotecnologia Marinha. Laboratório de Genética Marinha. Arraial do Cabo, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vorologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vorologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Norovirus | pt_BR |
| Subject | Oysters | pt_BR |
| Subject | Mussels | pt_BR |
| Subject | PMA treatment | pt_BR |
| Subject | Marine reserve | pt_BR |
| Subject | Rio de Janeiro | pt_BR |
| Embargo date | 2023 | |
| xmlui.metadata.dc.subject.ods | 14 Vida na água | |
