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ANTI-LEISHMANIA IGG IS A MARKER OF DISSEMINATED LEISHMANIASIS CAUSED BY LEISHMANIA BRAZILIENSIS
Disseminated leishmaniasis
Leishmania braziliensis
IgG
IgG2
Disease severity
Author
Affilliation
Abstract
In this study we determine the accuracy of anti-Leishmania IgG
and IgG subclasses to distinguish clinical forms of American tegumentary
leishmaniasis (ATL), and the relationship between antibodies levels with cytokine
production and severity of ATL.
Methods: Participants were 40 cutaneous leishmaniasis (CL), 20 mucosal
leishmaniasis (ML), 20 disseminated leishmaniasis (DL) patients and 20 subjects
with subclinical L. braziliensis infection (SC). Diagnosis was performed by DNA
of L. braziliensis or IFN-γ production in SC. IgG and subclasses of IgG to soluble
Leishmania antigen, as well cytokine levels in supernatants of mononuclear cells
were detected by ELISA.
Results: IgG were detected in 95%, 95% and 100% of CL, ML and DL patients,
respectively. Higher levels of anti-Leishmania IgG and IgG2 were seen in DL
compared to CL, ML and SC. ROC analysis confirmed the ability of IgG to
distinguish DL from the other clinical forms. A direct correlation was observed
between IgG titers and levels of IFN-γ and CXCL10 in CL and DL, and IgG2
antibodies correlated with the number of lesions in DL.
Conclusions: High Anti-Leishmania IgG and IgG2 levels are characteristic of DL,
and while IgG was correlated with pro-inflammatory cytokines, IgG2 was direct
correlated with number of lesions.
Keywords
Cutaneous leishmaniasisDisseminated leishmaniasis
Leishmania braziliensis
IgG
IgG2
Disease severity
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