Author | Rizk, Yasmin Silva | |
Author | Pereira, Sandy Santos | |
Author | Gervazoni, Luiza | |
Author | Hardoim, Daiana de Jesus | |
Author | Cardoso, Flávia de Oliveira | |
Author | Souza, Celeste da Silva Freitas | |
Author | Pelajo-Machado, Marcelo | |
Author | Carollo, Carlos Alexandre | |
Author | Arruda, Carla Cardozo Pinto de | |
Author | Amaral, Elmo Eduardo Almeida | |
Author | Valle, Tânia Zaverucha do | |
Author | Calanbrese, Kátia da Silva | |
Access date | 2021-04-16T19:04:35Z | |
Available date | 2021-04-16T19:04:35Z | |
Document date | 2021 | |
Citation | RIZK, Yasmin Silva et al. Amentoflavone as an Ally in the Treatment of Cutaneous Leishmaniasis: Analysis of Its Antioxidant/Prooxidant Mechanisms. Front. Cell. Infect. Microbiol., v. 11, Article 615814, 13p, Feb. 2021. | pt_BR |
ISSN | 2235-2988 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/46742 | |
Language | eng | pt_BR |
Publisher | Frontiers Media | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Amentoflavone | pt_BR |
Subject in Portuguese | Biflavonóide | pt_BR |
Subject in Portuguese | Prooxidante | pt_BR |
Subject in Portuguese | Atividade antileishmana | pt_BR |
Subject in Portuguese | Tratamento intralesional | pt_BR |
Subject in Portuguese | Leishmaniose cutânea | pt_BR |
Subject in Portuguese | Glucantime | pt_BR |
Title | Amentoflavone as an Ally in the Treatment of Cutaneous Leishmaniasis: Analysis of Its Antioxidant/Prooxidant Mechanisms | pt_BR |
Type | Article | pt_BR |
DOI | 10.3389/fcimb.2021.615814 | |
Abstract | Treatment of leishmaniasis is a challenging subject. Although available, chemotherapy is
limited, presenting toxicity and adverse effects. New drugs with antileishmanial activity are
being investigated, such as antiparasitic compounds derived from plants. In this work, we
investigated the antileishmanial activity of the biflavonoid amentoflavone on the protozoan
Leishmania amazonensis. Although the antileishmanial activity of amentoflavone has
already been reported in vitro, the mechanisms involved in the parasite death, as well
as its action in vivo, remain unknown. Amentoflavone demonstrated activity on intracellular
amastigotes in macrophages obtained from BALB/c mice (IC50 2.3 ± 0.93 mM). No
cytotoxicity was observed and the selectivity index was estimated as greater than 10.
Using BALB/c mice infected with L. amazonensis we verified the effect of an intralesional
treatment with amentoflavone (0.05 mg/kg/dose, in a total of 5 doses every 4 days).
Parasite quantification demonstrated that amentoflavone reduced the parasite load in
treated footpads (46.3% reduction by limiting dilution assay and 56.5% reduction by Real
Time Polymerase Chain Reaction). Amentoflavone decreased the nitric oxide production
in peritoneal macrophages obtained from treated animals. The treatment also increased
the expression of ferritin and decreased iNOS expression at the site of infection.
Furthemore, it increased the production of ROS in peritoneal macrophages infected in
vitro. The increase of ROS in vitro, associated with the reduction of NO and iNOS
expression in vivo, points to the antioxidant/prooxidant potential of amentoflavone, which
may play an important role in the balance between inflammatory and anti-inflammatory
patterns at the infection site. Taken together these results suggest that amentoflavone has the potential to be used in the treatment of cutaneous leishmaniasis, working as an ally in
the control and development of the lesion. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal de Mato Grosso do Sul. Instituto de Biociências. Laboratório de Produtos Naturais e Espectrometria de Massas. Campo Grande, MS, Brasil. | pt_BR |
Affilliation | Universidade Federal de Mato Grosso do Sul. Instituto de Biociências. Laboratório de Parasitologia Humana. Campo Grande, MS, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | undação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | undação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Amentoflavone | pt_BR |
Subject | Biflavonoid | pt_BR |
Subject | Prooxidant | pt_BR |
Subject | Antileishmanial activity | pt_BR |
Subject | Intralesional treatment | pt_BR |
Subject | Cutaneous leishmaniasis | pt_BR |
Subject | Glucantime | pt_BR |