| Author | Bandeira, Derick Mendes | |
| Author | Caldas, Gabriela Cardoso | |
| Author | Jácome, Fernanda Cunha | |
| Author | Rasinhas, Arthur da Costa | |
| Author | Almeida, Ana Luisa Teixeira de | |
| Author | Tourinho, Renata Santos | |
| Author | Amorim, Juliana Fernandes da Silva | |
| Author | Trindade, Gisela Freitas | |
| Author | Barth, Ortrud Monika | |
| Author | Vieira, Debora Ferreira Barreto | |
| Access date | 2021-04-25T18:24:26Z | |
| Available date | 2021-04-25T18:24:26Z | |
| Document date | 2021 | |
| Citation | BANDEIRA, Derick Mendes et al. Clinical aspects and detection of Zika virus RNA in several tissues of experimentally infected BALB/c mice, Resarch Square, p. 1-11, 2021. | |
| URI | https://www.arca.fiocruz.br/handle/icict/46878 | |
| Language | eng | en_US |
| Publisher | Resarch Square | |
| Rights | open access | |
| Subject in Portuguese | Zika virus RNA | pt_BR |
| Subject in Portuguese | Detecção do RNA | pt_BR |
| Subject in Portuguese | Aspectos clínicos | pt_BR |
| Subject in Portuguese | Tecidos de BALB/c mice | pt_BR |
| Subject in Portuguese | Virologia | pt_BR |
| Subject in Portuguese | qRT-PCR | pt_BR |
| Title | Clinical aspects and detection of Zika virus RNA in several tissues of experimentally infected BALB/c mice | en_US |
| Type | Preprint | |
| DOI | 10.34119/bjhrv4n1-160 | |
| Abstract | The aim of the present study was to infect BALB/c mice with ZIKV to evaluate clinical signs and to quantify the viral load in different tissues at three different kinetic points. For this purpose, fifteen mice were inoculated with a 100µl of a viral solution and five other mice were used as negative controls. After euthanasia, nine different tissues were collected and macerated for RNA extraction and quantification. The infections were not lethal. Some of them, however, showed great agitation, hair bristling and itchy skin. Viral RNA was detected in one heart sample, eight samples of spleen and two of skeletal muscle. Seven out of eleven positive samples were from mice euthanized on the third day after infection. Only spleen yielded positive results at a later time of infection. These results contribute to a better understanding of clinical signs and replication dynamics of Zika virus in different organs of BALB/c mice, which are still scarce data in the scientific literature. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | |
| Subject | Detection of Zika virus RNA | en_US |
| Subject | Clinical aspects | en_US |
| Subject | BALB/c mice | en_US |
| Subject | Tissues | en_US |
| Subject | qRT-PCR | en_US |
| Subject | Virology | en_US |
