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CLINICAL ASPECTS AND DETECTION OF ZIKA VIRUS RNA IN SEVERAL TISSUES OF EXPERIMENTALLY INFECTED BALB/C MICE
Detecção do RNA
Aspectos clínicos
Tecidos de BALB/c mice
Virologia
qRT-PCR
Author
Affilliation
Abstract
The aim of the present study was to infect BALB/c mice with ZIKV to evaluate clinical signs and to quantify the viral load in different tissues at three different kinetic points. For this purpose, fifteen mice were inoculated with a 100µl of a viral solution and five other mice were used as negative controls. After euthanasia, nine different tissues were collected and macerated for RNA extraction and quantification. The infections were not lethal. Some of them, however, showed great agitation, hair bristling and itchy skin. Viral RNA was detected in one heart sample, eight samples of spleen and two of skeletal muscle. Seven out of eleven positive samples were from mice euthanized on the third day after infection. Only spleen yielded positive results at a later time of infection. These results contribute to a better understanding of clinical signs and replication dynamics of Zika virus in different organs of BALB/c mice, which are still scarce data in the scientific literature.
Keywords in Portuguese
Zika virus RNADetecção do RNA
Aspectos clínicos
Tecidos de BALB/c mice
Virologia
qRT-PCR
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