Author | Zamith-Miranda, Daniel | |
Author | Silva, Roberta Peres da | |
Author | Couvillion, Sneha P. | |
Author | Bredeweg, Erin L. | |
Author | Burnet, Meagan C. | |
Author | Coelho, Carolina | |
Author | Camacho, Emma | |
Author | Nimrichter, Leonardo | |
Author | Puccia, Rosana | |
Author | Almeida, Igor C. | |
Author | Casadevall, Arturo | |
Author | Rodrigues, Marcio Lourenço | |
Author | Alves, Lysangela Ronalte | |
Author | Nosanchuk, Joshua D. | |
Author | Nakayasu, Ernesto S. | |
Access date | 2021-06-11T13:52:33Z | |
Available date | 2021-06-11T13:52:33Z | |
Document date | 2021 | |
Citation | ZAMITH-MIRANDA, Daniel et al. Omics approaches for understanding biogenesis, composition and functions of fungal extracellular vesicles. Frontiers in Genetics, v. 12, p. 1–16, 2021. | pt_BR |
ISSN | 1664-8021 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/47646 | |
Language | por | pt_BR |
Publisher | Frontiers Media | pt_BR |
Rights | open access | pt_BR |
Title | Omics approaches for understanding biogenesis, composition and functions of fungal extracellular vesicles | pt_BR |
Type | Article | pt_BR |
DOI | 10.3389/fgene.2021.648524 | |
Abstract | Extracellular vesicles (EVs) are lipid bilayer structures released by organisms from all kingdoms of life. The diverse biogenesis pathways of EVs result in a wide variety of physical properties and functions across different organisms. Fungal EVs were first described in 2007 and different omics approaches have been fundamental to understand their composition, biogenesis, and function. In this review, we discuss the role of omics in elucidating fungal EVs biology. Transcriptomics, proteomics, metabolomics, and lipidomics have each enabled the molecular characterization of fungal EVs, providing evidence that these structures serve a wide array of functions, ranging from key carriers of cell wall biosynthetic machinery to virulence factors. Omics in combination with genetic approaches have been instrumental in determining both biogenesis and cargo loading into EVs. We also discuss how omics technologies are being employed to elucidate the role of EVs in antifungal resistance, disease biomarkers, and their potential use as vaccines. Finally, we review recent advances in analytical technology and multi-omic integration tools, which will help to address key knowledge gaps in EVs biology and translate basic research information into urgently needed clinical applications such as diagnostics, and immuno- and chemotherapies to fungal infections. | pt_BR |
Affilliation | Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, NY, USA / Division of Infectious Diseases. Department of Medicine. Albert Einstein College of Medicine. Bronx, NY, USA. | pt_BR |
Affilliation | Centre for Medical Mycology. University of Exeter, Exeter, United Kingdom. | pt_BR |
Affilliation | Biological Sciences Division. Pacific Northwest National Laboratory. Richland, WA, USA. | pt_BR |
Affilliation | Environmental and Molecular Sciences Laboratory. Pacific Northwest National Laboratory. Richland, WA, USA. | pt_BR |
Affilliation | Biological Sciences Division. Pacific Northwest National Laboratory. Richland, WA, USA. | pt_BR |
Affilliation | Centre for Medical Mycology. University of Exeter, Exeter, United Kingdom. | pt_BR |
Affilliation | Department of Molecular Microbiology and Immunology. Johns Hopkins Bloomberg School of Public Health. Baltimore, MD, USA. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Glicobiologia de Eucariotos. Rio de Janeiro RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Department of Biological Sciences. Border Biomedical Research Center. University of Texas at El Paso. El Paso, TX, USA. | pt_BR |
Affilliation | Department of Molecular Microbiology and Immunology. Johns Hopkins Bloomberg School of Public Health. Baltimore, MD, USA. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, NY, USA / Division of Infectious Diseases. Department of Medicine. Albert Einstein College of Medicine. Bronx, NY, USA. | pt_BR |
Affilliation | Biological Sciences Division. Pacific Northwest National Laboratory. Richland, WA, USA. | pt_BR |
Subject | Extracellular Vesicles | pt_BR |
Subject | Fungi | pt_BR |
Subject | Virulence | pt_BR |
Subject | Systems Biology | pt_BR |
Subject | Proteomics | pt_BR |
Subject | Metabolomics | pt_BR |
Subject | Lipidomics | pt_BR |
Subject | Transcriptomics | pt_BR |
Subject in Spanish | Vesículas Extracelulares | pt_BR |
Subject in Spanish | Hongos | pt_BR |
Subject in Spanish | Virulencia | pt_BR |
Subject in Spanish | Biología de Sistemas | pt_BR |
Subject in Spanish | Proteómica | pt_BR |
Subject in Spanish | Metabolómica | pt_BR |
Subject in Spanish | Lipidómica | pt_BR |
Subject in Spanish | Transcriptoma | pt_BR |
Subject in French | Vésicules extracellulaires | pt_BR |
Subject in French | Champignons | pt_BR |
Subject in French | Biologie des systèmes | pt_BR |
Subject in French | Protéomique | pt_BR |
Subject in French | Métabolomique | pt_BR |
DeCS | Vesículas Extracelulares | pt_BR |
DeCS | Fungos | pt_BR |
DeCS | Virulência | pt_BR |
DeCS | Biologia de Sistemas | pt_BR |
DeCS | Proteômica | pt_BR |
DeCS | Metabolômica | pt_BR |
DeCS | Lipidômica | pt_BR |
DeCS | Transcriptome | pt_BR |