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https://www.arca.fiocruz.br/handle/icict/47646
OMICS APPROACHES FOR UNDERSTANDING BIOGENESIS, COMPOSITION AND FUNCTIONS OF FUNGAL EXTRACELLULAR VESICLES
Fungi
Virulence
Systems Biology
Proteomics
Metabolomics
Lipidomics
Transcriptomics
Hongos
Virulencia
Biología de Sistemas
Proteómica
Metabolómica
Lipidómica
Transcriptoma
Fungos
Virulência
Biologia de Sistemas
Proteômica
Metabolômica
Lipidômica
Transcriptome
Author
Zamith-Miranda, Daniel
Silva, Roberta Peres da
Couvillion, Sneha P.
Bredeweg, Erin L.
Burnet, Meagan C.
Coelho, Carolina
Camacho, Emma
Nimrichter, Leonardo
Puccia, Rosana
Almeida, Igor C.
Casadevall, Arturo
Rodrigues, Marcio Lourenço
Alves, Lysangela Ronalte
Nosanchuk, Joshua D.
Nakayasu, Ernesto S.
Silva, Roberta Peres da
Couvillion, Sneha P.
Bredeweg, Erin L.
Burnet, Meagan C.
Coelho, Carolina
Camacho, Emma
Nimrichter, Leonardo
Puccia, Rosana
Almeida, Igor C.
Casadevall, Arturo
Rodrigues, Marcio Lourenço
Alves, Lysangela Ronalte
Nosanchuk, Joshua D.
Nakayasu, Ernesto S.
Affilliation
Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, NY, USA / Division of Infectious Diseases. Department of Medicine. Albert Einstein College of Medicine. Bronx, NY, USA.
Centre for Medical Mycology. University of Exeter, Exeter, United Kingdom.
Biological Sciences Division. Pacific Northwest National Laboratory. Richland, WA, USA.
Environmental and Molecular Sciences Laboratory. Pacific Northwest National Laboratory. Richland, WA, USA.
Biological Sciences Division. Pacific Northwest National Laboratory. Richland, WA, USA.
Centre for Medical Mycology. University of Exeter, Exeter, United Kingdom.
Department of Molecular Microbiology and Immunology. Johns Hopkins Bloomberg School of Public Health. Baltimore, MD, USA.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Glicobiologia de Eucariotos. Rio de Janeiro RJ, Brasil.
Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
Department of Biological Sciences. Border Biomedical Research Center. University of Texas at El Paso. El Paso, TX, USA.
Department of Molecular Microbiology and Immunology. Johns Hopkins Bloomberg School of Public Health. Baltimore, MD, USA.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.
Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, NY, USA / Division of Infectious Diseases. Department of Medicine. Albert Einstein College of Medicine. Bronx, NY, USA.
Biological Sciences Division. Pacific Northwest National Laboratory. Richland, WA, USA.
Centre for Medical Mycology. University of Exeter, Exeter, United Kingdom.
Biological Sciences Division. Pacific Northwest National Laboratory. Richland, WA, USA.
Environmental and Molecular Sciences Laboratory. Pacific Northwest National Laboratory. Richland, WA, USA.
Biological Sciences Division. Pacific Northwest National Laboratory. Richland, WA, USA.
Centre for Medical Mycology. University of Exeter, Exeter, United Kingdom.
Department of Molecular Microbiology and Immunology. Johns Hopkins Bloomberg School of Public Health. Baltimore, MD, USA.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Glicobiologia de Eucariotos. Rio de Janeiro RJ, Brasil.
Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.
Department of Biological Sciences. Border Biomedical Research Center. University of Texas at El Paso. El Paso, TX, USA.
Department of Molecular Microbiology and Immunology. Johns Hopkins Bloomberg School of Public Health. Baltimore, MD, USA.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.
Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, NY, USA / Division of Infectious Diseases. Department of Medicine. Albert Einstein College of Medicine. Bronx, NY, USA.
Biological Sciences Division. Pacific Northwest National Laboratory. Richland, WA, USA.
Abstract
Extracellular vesicles (EVs) are lipid bilayer structures released by organisms from all kingdoms of life. The diverse biogenesis pathways of EVs result in a wide variety of physical properties and functions across different organisms. Fungal EVs were first described in 2007 and different omics approaches have been fundamental to understand their composition, biogenesis, and function. In this review, we discuss the role of omics in elucidating fungal EVs biology. Transcriptomics, proteomics, metabolomics, and lipidomics have each enabled the molecular characterization of fungal EVs, providing evidence that these structures serve a wide array of functions, ranging from key carriers of cell wall biosynthetic machinery to virulence factors. Omics in combination with genetic approaches have been instrumental in determining both biogenesis and cargo loading into EVs. We also discuss how omics technologies are being employed to elucidate the role of EVs in antifungal resistance, disease biomarkers, and their potential use as vaccines. Finally, we review recent advances in analytical technology and multi-omic integration tools, which will help to address key knowledge gaps in EVs biology and translate basic research information into urgently needed clinical applications such as diagnostics, and immuno- and chemotherapies to fungal infections.
Keywords
Extracellular VesiclesFungi
Virulence
Systems Biology
Proteomics
Metabolomics
Lipidomics
Transcriptomics
Keywords in Spanish
Vesículas ExtracelularesHongos
Virulencia
Biología de Sistemas
Proteómica
Metabolómica
Lipidómica
Transcriptoma
DeCS
Vesículas ExtracelularesFungos
Virulência
Biologia de Sistemas
Proteômica
Metabolômica
Lipidômica
Transcriptome
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