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IMMUNE OUTCOMES OF ZIKA VIRUS INFECTION IN NONHUMAN PRIMATES
Schouest, Blake et al. | Date Issued: 2020
Author
Schouest, Blake
Fahlberg, Marissa
Scheef, Elizabeth A.
Ward, Matthew J.
Headrick, Kyra
Szeltner, Dawn M.
Blair, Robert V.
Gilbert, Margaret H.
Doyle‑Meyers, Lara A.
Danner, Victoria W.
Bonaldo, Myrna C.
Wesson, Dawn M.
Panganiban, Antonito T.
Maness, Nicholas J.
Affilliation
Biomedical Sciences Training Program, Tulane University School of Medicine, New Orleans, LA, USA / Division of Microbiology, Tulane National Primate Research Center, Covington, LA, USA.
Division of Immunology, Tulane National Primate Research Center, Covington, LA, USA.
Division of Microbiology, Tulane National Primate Research Center, Covington, LA, USA.
School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA, USA.
School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA, USA.
Division of Microbiology, Tulane National Primate Research Center, Covington, LA, USA.
Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA, USA.
Division of Veterinary Medicine, Tulane National Primate Research Center, Covington, LA, USA.
Division of Veterinary Medicine, Tulane National Primate Research Center, Covington, LA, USA.
Division of Veterinary Medicine, Tulane National Primate Research Center, Covington, LA, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.
School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA, USA.
Division of Microbiology, Tulane National Primate Research Center, Covington, LA, USA / Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA, USA.
Division of Microbiology, Tulane National Primate Research Center, Covington, LA, USA / Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA, USA.
Abstract
Although the Zika virus (ZIKV) epidemic is subsiding, immune responses that are important for controlling acute infection have not been defnitively characterized. Nonhuman primate (NHP) models were rapidly developed to understand the disease and to test vaccines, and these models have since provided an understanding of the immune responses that correlate with protection during natural infection and vaccination. Here, we infected a small group of male rhesus (Macaca mulatta) and cynomolgus (Macaca fascicularis) macaques with a minimally passaged Brazilian ZIKV isolate and used multicolor fow cytometry and transcriptional profling to describe early immune patterns following infection. We found evidence of strong innate antiviral responses together with induction of neutralizing antibodies and T cell responses. We also assessed the relative importance of CD8 T cells in controlling infection by carrying out CD8 T cell depletion in an additional two animals of each species. CD8 depletion appeared to dysregulate early antiviral responses and possibly increase viral persistence, but the absence of CD8 T cells ultimately did not impair control of the virus. Together, these data describe immunological trends in two NHP species during acute ZIKV infection, providing an account of early responses that may be important in controlling infection.
Keywords in Portuguese
Vírus da Zika
Resultados imunes
Infecção
Primatas não humanos
 
Keywords
Zika virus
Infection
Nonhuman primates
Immune outcomes
 
Publisher
Nature Research
Citation
SCHOUEST, Blake et al. Immune outcomes of Zika virus infection in nonhuman primates. Scientific Reports, v. 10, 13069, p. 1-12, 2020.
DOI
10.1038/s41598-020-69978-w
ISSN
2045-2322