Background: Botanical substances such as essential oils (EOs) have demonstrated insecticidal properties and are a valid option for vector control. However, free EOs are unreliable as mosquito larvicides due their easy degradation by environmental exposure to ultraviolet light and higher temperatures. Here, we assessed the efcacy of a mosquito larvicide based on orange oil in a yeast-based delivery system against Aedes aegypti strains with diferent resistance status towards chemical neurotoxic insecticides. This larvicide preparation was physicochemically characterized in a previous report. Methods: Larvae of four Ae. aegypti strains from diferent regions of Brazil and diferent resistance profles for del‑ tamethrin (pyrethroid) and temephos (organophosphate) were tested against yeast-encapsulated orange oil (YEOO) in laboratory conditions for measurement of LC50 and LC90 values. The same assays were performed with the Belo Horizonte strain under environmental conditions (natural light and temperature). The resistance profles of these strains were compared to the Rockefeller reference strain in all conditions. Results: YEOO was found to be a highly active larvicide (LC50<50 mg/L) against all Ae. aegypti strains tested in both laboratory conditions (LC50=8.1–24.7 mg/L) and environmental conditions with natural light and temperature fuctuation (LC50=20.0–49.9 mg/L). Moreover, all strains were considered susceptible (RR<5) to YEOO, consider‑ ing resistance ratios calculated based on the Rockefeller strain. The resistance ratios were only higher than 2.5 for LC90–95 of Belo Horizonte in the laboratory, probably due the higher heterogeneity associated with older egg papers (>5 months). Conclusion: YEOO demonstrates high larvicidal activity against Ae. aegypti strains with resistant phenotypes for deltamethrin (PY) and temephos (OP). This larvicidal activity suggests the potential for the development of YEOO as an alternative intervention to synthetic insecticides in integrated vector management programs, for populations with resistance to commonly used insecticides.