Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/48330
Title: Antibodies response induced by recombinant virus-like particles from Triatoma virus and chimeric antigens from Trypanosoma cruzi
Authors: Queiroz, Aline Maria Vasconcelos
Yanshina, Yulia Aleksandrovna
Rodrigues, Emily Thays da Silva
Santos, Fred Luciano Neves
Celedon, Paola Alejandra Fiorani
Maheshwari, Sweta
Gabelli, Sandra Beatriz
Rubio, Carla Stephanie Peucelle
Durana, Aritz
Guérin, Diego M. A.
Silva, Marcelo Sousa
Affilliation: Universidade Federal do Rio Grande do Norte. Centro de Ciências da Saúde. Programa de Pós-Graduação em Ciências Farmacêuticas. Natal, RN, Brasil / Universidade Federal do Rio Grande do Norte. Centro de Ciências da Saúde. Departamento de Análises Clínicas e Toxicológicas. Laboratório de Imunoparasitologia. Natal, RN, Brasil.
Universidade Nova de Lisboa. Instituto de Higiene e Medicina Tropical. Programa Internacional de Doutoramento em Doenças Tropicais e Saúde Global. Lisboa, Portugal.
Universidade Federal do Rio Grande do Norte. Centro de Ciências da Saúde. Departamento de Análises Clínicas e Toxicológicas. Laboratório de Imunoparasitologia. Natal, RN, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.
Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
Johns Hopkins University. School of Medicine. Baltimore, MD, USA.
Johns Hopkins University. School of Medicine. Baltimore, MD, USA.
Universidad del País Vasco. Instituto Biofisika. Departamento de Bioquímica y Biología Molecular. Leioa, Bizkaia, España / Ikosaedrika Biologicals. Vizcaya, España.
Fundación Biofísica Bizkaia. Instituto Biofisika. Leioa, Vizcaya, España.
Universidad del País Vasco. Instituto Biofisika. Departamento de Bioquímica y Biología Molecular. Leioa, Bizkaia, España / Ikosaedrika Biologicals. Vizcaya, España.
Universidade Federal do Rio Grande do Norte. Centro de Ciências da Saúde. Programa de Pós-Graduação em Ciências Farmacêuticas. Natal, RN, Brasil / Universidade Federal do Rio Grande do Norte. Centro de Ciências da Saúde. Departamento de Análises Clínicas e Toxicológicas. Laboratório de Imunoparasitologia. Natal, RN, Brasil / Universidade Nova de Lisboa. Instituto de Higiene e Medicina Tropical. Lisboa, Portugal.
Abstract: Background: The infection caused by the protozoan Trypanosoma cruzi affects humans and is called as Chagas disease. Currently, the main measures available to reduce the incidence of this disease are drug treatment and vector control. Traditionally, the development of vaccines occurs mainly through the use of antigenic candidates of the etiologic agent in the form of a vaccine preparation. Virus-like particles (VLPs) are structures analogous to viral capsids composed essentially of structural proteins and are widely used in vaccination protocols because of their immunostimulatory properties. In this context, the objective of this study was to use strategies in a murine immunization model to characterize the immunostimulatory capacity of VLPs from Triatoma virus (TrV-VLPs), analysed in the presence or absence of the aluminium vaccine adjuvant. In parallel, to characterize the immunogenic behaviour of four T. cruzi chimeric recombinant proteins (mix-IBMP) associated with TrV-VLPs or aluminium vaccine adjuvant. Method: We immunized BALB/c mice once or twice, depending on the strategy, and collected serum samples at 15, 30 and 45 days after the immunization. Subsequently, serum samples from animals immunized with TrV-VLPs were used to determine total IgG, IgG1, IgG2a, IgG2b and IgG3 anti-TrV-VLPs by enzyme-linked immunosorbent assay (ELISA). Results: Data obtained demonstrate the ability of TrV-VLPs to preferably induce IgG2b and IgG3 type antibodies in the absence of aluminium adjuvant. In fact, the use of aluminium did not interfere with the total IgG profile of anti-TrV-VLPs. Interestingly, mix-IBMP had a better profile of total IgG, IgG1 and IgG3 subclasses when mixed with TrV-VLPs. Conclusion: In conclusion, these results suggest the potential of TrV-VLPs as a vaccine adjuvant and the use of T. cruzi chimeric antigens as a rational strategy for the development of vaccines against the experimental model of Chagas disease.
Keywords: Virus-like particles (VLPs)
Chagas disease
Trypanosoma cruzi
Adjuvant
Humoral immune response
Triatoma virus
keywords: Vírus
Doença de Chagas
Trypanosoma cruzi
Vacinação
Imunização
Triatoma
Issue Date: 2021
Publisher: Elsevier
Citation: QUEIROZ, Aline Maria Vasconcelos et al. Antibodies response induced by recombinant virus-like particles from Triatoma virus and chimeric antigens from Trypanosoma cruzi. Vaccine, v. 39, n. 33, p. 4723-4732, 30 July 2021.
DOI: 10.1016/j.vaccine.2021.05.039
ISSN: 0264-410X
Copyright: restricted access
Appears in Collections:BA - IGM - Artigos de Periódicos
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