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CANDIDA GLABRATA PRODUCES A MELANIN-LIKE PIGMENT THAT PROTECTS AGAINST STRESS CONDITIONS ENCOUNTERED DURING PARASITISM
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Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil / Albert Einstein College of Medicine. Departments of Medicine (Division of Infectious Diseases) & Microbiology & Immunology. Bronx, NY, USA.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Albert Einstein College of Medicine. Departments of Medicine (Division of Infectious Diseases) & Microbiology & Immunology. Bronx, NY, USA / Universidade de São Paulo. Departamento de Microbiologia. Instituto de Ciências Biomédicas. São Paulo, SP, Brasil.
Albert Einstein College of Medicine. Department of Biophysics. Bronx, NY, USA.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Biofísica de Fungos. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Biofísica de Fungos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Albert Einstein College of Medicine. Departments of Medicine (Division of Infectious Diseases) & Microbiology & Immunology. Bronx, NY, USA.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Albert Einstein College of Medicine. Departments of Medicine (Division of Infectious Diseases) & Microbiology & Immunology. Bronx, NY, USA / Universidade de São Paulo. Departamento de Microbiologia. Instituto de Ciências Biomédicas. São Paulo, SP, Brasil.
Albert Einstein College of Medicine. Department of Biophysics. Bronx, NY, USA.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Biofísica de Fungos. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Biofísica de Fungos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Albert Einstein College of Medicine. Departments of Medicine (Division of Infectious Diseases) & Microbiology & Immunology. Bronx, NY, USA.
Abstract
Aim: Melanin has been linked to pathogenesis in several fungi. They often produce melanin-like pigments in the presence of L-dihydroxyphenylalanine (L-DOPA), but this is poorly studied in Candida glabrata. Methods & materials: C. glabrata was grown in minimal medium with or without L-DOPA supplementation and submitted to a chemical treatment with denaturant and hot acid. Results: C. glabrata turned black when grown in the presence of L-DOPA, whereas cells grown without L-DOPA supplementation remained white. Biophysical properties demonstrated that the pigment was melanin. Melanized C. glabrata cells were effectively protected from azoles and amphotericin B, incubation at 42°C and macrophage killing. Conclusion: In the presence of L-DOPA, C. glabrata produces melanin, increases antifungal resistance and enhances host survival.
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