Please use this identifier to cite or link to this item:
ASSESSING THE ANTIGENICITY OF DIFFERENT VP3 REGIONS OF INFECTIOUS BURSAL DISEASE VIRUS IN CHICKENS FROM SOUTH BRAZIL
Gumboro disease
VP3
Viral Structural Proteins
Antigenic regions
Immunodominant Epitopes
Enzyme-Linked Immunosorbent Assay
Proteínas Estructurales Virales
Epítopos Inmunodominantes
Ensayo de Inmunoadsorción Enzimática
Proteínas Estruturais Virais
Epitopos Imunodominantes
Ensaio de Imunoadsorção Enzimática
Author
Affilliation
Abstract
Infectious bursal disease (IBD), also known as Gumboro disease, is a viral infection that causes mortality and immunosuppression in chickens (Gallus gallus). VP2 and VP3 are the major structural viral capsid components and are the most immunogenic proteins of IBD virus (IBDV). Reliable diagnostic tests using VP2 and VP3 produced in heterologous systems are important tools to control this infection. One advantage of an IBD diagnostic based on VP3, over those that use VP2, is that VP3 has linear epitopes, enabling its production in bacteria. We tested the suitability of recombinant VP3 (rVP3) as a diagnostic reagent in an enzyme-linked immunosorbent assay (ELISA). Compared with a commercial test, rVP3 ELISA showed high sensitivity and specificity as a diagnostic tool for vaccinated animals. In addition, rVP3, but not the commercial ELISA, was able to detect antibodies in nonvaccinated chickens, probably developed against circulating IBDV strains. It was possible the assessment of VP3 regions antigenicity using chicken antisera. The full-length recombinant VP3 can be used to assess post vaccination immunological status of chickens and its production is feasible and inexpensive. The evaluation of VP3 regions as candidates for general use in the diagnosis of IBD in chickens should be conducted with caution. Our work was the first to identify several regions of VP3 recognized by chicken antibodies
Keywords
Infectious bursal disease virusGumboro disease
VP3
Viral Structural Proteins
Antigenic regions
Immunodominant Epitopes
Enzyme-Linked Immunosorbent Assay
Keywords in Spanish
Virus de la Enfermedad Infecciosa de la BolsaProteínas Estructurales Virales
Epítopos Inmunodominantes
Ensayo de Inmunoadsorción Enzimática
DeCS
Vírus da Doença Infecciosa da BursaProteínas Estruturais Virais
Epitopos Imunodominantes
Ensaio de Imunoadsorção Enzimática
Share