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PHYTOCHEMICAL CHARACTERIZATION AND ANTIBIOTIC POTENTIATING EFFECTS OF THE ESSENTIAL OIL OF ALOYSIA GRATISSIMA (GILLIES & HOOK.) AND BETA-CARYOPHYLLENE
Espectrometria de Massas
Resistência bacteriana
Bombas
Ciprofloxacina
Author
Santos, Eduardo L.
Freitas, Priscilla R.
Araujo, Ana Carolina J.
Almeida, Ray S.
Tintino, Saulo R.
Paulo, Cicera Laura R.
Ribeiro Filho, Jaime
Silva, Ana Cristina A.
Silva, Luiz E
Amaral, Wanderlei do
Deschampse, Cícero
Siqueira Junior, Jose Pinto
Barbosa Filho, Jose Maria
Sousa, Gabriela Ribeiro de
Coutinho, Henrique D.M.
Freitas, Priscilla R.
Araujo, Ana Carolina J.
Almeida, Ray S.
Tintino, Saulo R.
Paulo, Cicera Laura R.
Ribeiro Filho, Jaime
Silva, Ana Cristina A.
Silva, Luiz E
Amaral, Wanderlei do
Deschampse, Cícero
Siqueira Junior, Jose Pinto
Barbosa Filho, Jose Maria
Sousa, Gabriela Ribeiro de
Coutinho, Henrique D.M.
Affilliation
"Múltipla ver em Notas"
Abstract
The traditional knowledge associated with the use of medicinal plants has significantly contributed to drug
development research. In this context, Aloysia gratissima, commonly known as bee-brush or white-brush,
has been widely used in Brazilian folk medicine as an analgesic and antimicrobial species. Phytochemical
analysis of A. gratissima essential oil has identified sesquiterpene b-caryophyllene (17.3%) as a major component
with significant antibacterial activity. Thus, this study aimed to characterize the antibacterial effects of
the essential oil obtained from A. gratissima (EOAG) and its major compound b-caryophyllene, as well as to
evaluate their potential to act as efflux pumps inhibitors in association with conventional antibiotics. The
phytochemical characterization of EOAG was performed by gas chromatography coupled to mass spectrometry
(GC-MS), while the antibacterial activity against the strains Pseudomonas aeruginosa 24, Staphylococcus
aureus 10, and Escherichia coli 06 was assessed using the broth microdilution method. The antibiotic potentiating
activity was investigated by analyzing the ability of the natural products to decrease the minimum
inhibitory concentration (MIC) of norfloxacin and ciprofloxacin against S. aureus strains 1199B and K2068. A
reduction in the MIC of ethidium bromide in the presence of essential oil components was interpreted as
efflux pump inhibition. The GC-MS analysis identified 30 compounds, including b-caryophyllene as the
major component. The antibacterial activity analysis demonstrated that both EOAG and b-caryophyllene
both EOAG and b-caryophyllene presented antibacterial effects against S. aureus 10 (MIC = 32 mg/mL), in
addition to potentiating the activity of norfloxacin against [S. aureus 10], [P. aeruginosa 24], and [E. coli 06].
The isolated compound also reversed the degree of antibiotic resistance observed in strains carrying efflux
pumps. In conclusion, the essential oil of A. gratissima and b-caryophyllene have the potential to increase the
effectiveness of antibiotics and as such can be used in the development of new therapies against bacterial
resistance.
Keywords in Portuguese
Pseudomonas aeruginosaEspectrometria de Massas
Resistência bacteriana
Bombas
Ciprofloxacina
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