Introduction: This short communication presents a novel report on the occurrence of Panstrongylus rufotuberculatus in the Brazilian state of Rondônia. Methods: Two specimens were collected inside dwellings and identified using dichotomous keys. Results: The present study showed the extensive geographic distribution of P. rufotuberculatus and the increased number of species in the state of Rondônia. Conclusions: This new record of P. rufotuberculatus is important for understanding the epidemiology of Chagas disease because this species is found naturally infected with Trypanosoma cruzi. Studies on the ecology, biology, and vector–host–parasite interactions of this species are essential for surveillance programs.