Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/50957
Title: Virome in roof-harvested rainwater of a densely urbanized low-income region
Authors: Prado, Tatiana
Shubo, Tatsuo
Freitas, Lucas
Leomil, Luciana
Maranhão, Adriana Gonçalves
Miagostovich, Marize Pereira
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.
Instituto SENAI de Inovação em Biossintéticos e Fibras, Centro Tecnológico da Indústria Química e Têxtil, SENAI CETIQT. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Abstract: Rainwater harvesting has been considered an affordable practice to supplement the conventional sources of water supply for potable and non-potable uses worldwide. This study characterizes the viral community found in roof-harvested rainwater (RHRW) samples obtained under different rain volumes in a densely urbanized low-income region in Rio de Janeiro, Brazil. Three pilot-scale standardized metal-sheet roofs (same catchment area, material age, and slope – 3%) were installed in the study area aiming at obtaining more reliable and representative samples. Fifty-four samples were collected fromsix rainfall events fromJanuary to April 2019 and concentrated by the skimmed-milk flocculation method. Pools of different rainfall volumes were submitted to high throughput sequencing using the shotgunmetagenomic approach. Sequencing was performed on NextSeq platform. Genomic analysis of the virus community revealed that most are RNA non-human viruses, including two main families: Dicistroviridae and Iflaviridae, recognized for infecting arthropods. Bacteriophages were also relatively abundant, with a predominance of DNA phages belonging to Microviridae and Siphoviridae families, showing percentages from5.3 and 3.7% of the total viral hits present in these samples, respectively. Viral genomic RNA viruses (77%) predominated over DNA viruses (23%). Concerning number of viral species identified, a higher percentage was observed for plant viruses (12 families, 58%). Hepatitis A virus and human klassevirus 1 were detected among the established human pathogens, suggesting the need for RHRW treatment before it is considered for human consumption. Australian bat lyssavirus was also detected, emphasizing the importance of environmentalmonitoring facing emerging viruses. The results corroborate the influence of the surrounding area on the rainwater quality.
Keywords: Reuse water
Roof-harvested rainwater (RHRW)
Rainfall volume
Virome
keywords: Reutilizar água
Água da chuva captada no telhado
Volume de chuva
Virome
Issue Date: 2022
Publisher: Elsevier
Citation: PRADO, Tatiana et al. Virome in roof-harvested rainwater of a densely urbanized low-income region. Science of the Total Environment. v. 807, 15078, p. 1 - 8, 2022.
DOI: 10.1016/j.scitotenv.2021.150778
ISSN: 0048-9697
Embargo date: 2023
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos
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