Please use this identifier to cite or link to this item:
Type
ArticleCopyright
Open access
Collections
- IOC - Artigos de Periódicos [12873]
Metadata
Show full item record
PERFORMANCE ASSESSMENT OF A MULTI‑EPITOPE CHIMERIC ANTIGEN FOR THE SEROLOGICAL DIAGNOSIS OF ACUTE MAYARO FEVER
Antígeno quimérico multiepítopo
Diagnóstico sorológico
Febre Mayaro aguda
Multi‑epitope chimeric antigen
Serological diagnosis
Acute Mayaro fever
Author
Affilliation
Abstract
Mayaro virus (MAYV), which causes mayaro fever, is endemic to limited regions of South America that
may expand due to the possible involvement of Aedes spp. mosquitoes in its transmission. Its effective
control will require the accurate identification of infected individuals, which has been restricted to
nucleic acid-based tests due to similarities with other emerging members of the Alphavirus genus
of the Togaviridae family; both in structure and clinical symptoms. Serological tests have a more
significant potential to expand testing at a reasonable cost, and their performance primarily reflects
that of the antigen utilized to capture pathogen-specific antibodies. Here, we describe the assembly of
a synthetic gene encoding multiple copies of antigenic determinants mapped from the nsP1, nsP2, E1,
and E2 proteins of MAYV that readily expressed as a stable chimeric protein in bacteria. Its serological
performance as the target in ELISAs revealed a high accuracy for detecting anti-MAYV IgM antibodies.
No cross-reactivity was observed with serum from seropositive individuals for dengue, chikungunya,
yellow fever, Zika, and other infectious diseases as well as healthy individuals. Our data suggest that
this bioengineered antigen could be used to develop high-performance serological tests for MAYV
infections.
Keywords in Portuguese
Avaliação de desempenhoAntígeno quimérico multiepítopo
Diagnóstico sorológico
Febre Mayaro aguda
Keywords
Performance assessmentMulti‑epitope chimeric antigen
Serological diagnosis
Acute Mayaro fever
Share