Title:	Peripheral blood iNKT cell activation correlates with liver damage during acute hepatitis C
Authors:	Senff, Tina Menne, Christopher Cosmovici, Christine Lewis-Ximenez, Lia Laura Aneja, Jasneet Broering, Ruth Kim, Arthur Y. Westendorf, Astrid M. Dittmer, Ulf Scherbaum, Norbert Lauer, Georg M. Timm, Jörg
Affilliation:	Institute of Virology, University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, Medical Faculty. Düsseldorf, Germany. Institute of Virology, University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, Medical Faculty. Düsseldorf, Germany. Institute of Virology, University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, Medical Faculty. Düsseldorf, Germany. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. Gastrointestinal Unit, Massachusetts General Hospital and Harvard Medical School. Boston, Massachusetts, USA. Department of Gastroenterology and Hepatology, University Hospital Essen, University of Duisburg-Essen. Essen, Germany. Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital, Boston. Massachusetts, USA. Institute of Medical Microbiology. University of Duisburg-Essen. Germany. Institute for Virology, University Hospital Essen. University of Duisburg-Essen, Germany. LVR-Hospital Essen, Department of Addictive Behaviour and Addiction Medicine, Medical Faculty. University of Duisburg- Essen, Essen, Germany. Gastrointestinal Unit, Massachusetts General Hospital and Harvard Medical School. Boston, Massachusetts, USA. Institute of Virology, University Hospital Düsseldorf, Heinrich Heine University Düsseldorf, Medical Faculty. Düsseldorf, Germany.
Abstract:	Invariant NK T (iNKT) cells are implicated in viral clearance; however, their role in hepatitis C virus (HCV) infection remains controversial. Here, iNKT cells were studied during different stages of HCV infection. iNKT cells from patients with acute HCV infection and people who inject drugs (PWID) with chronic or spontaneously resolved HCV infection were characterized by flow cytometry. In a longitudinal analysis during acute HCV infection, frequencies of activated CD38+ iNKT cells reproducibly declined in spontaneously resolving patients, whereas they were persistently elevated in patients progressing to chronic infection. During the first year of infection, the frequency of activated CD38+ or CD69+ iNKT cells strongly correlated with alanine transaminase levels with particularly pronounced correlations in spontaneously resolving patients. Increased frequencies of activated iNKT cells in chronic HCV infection were confirmed in cross-sectional analyses of PWID with chronic or spontaneously resolved HCV infection; however, no apparent functional differences were observed with various stimulation protocols. Our data suggest that iNKT cells are activated during acute hepatitis C and that activation is sustained in chronic infection. The correlation between the frequency of activated iNKT cells and alanine transaminase may point toward a role of iNKT cells in liver damage.
Keywords:	Peripheral blood iNKT cell activation Correlate Liver damage Acute hepatitis C
keywords:	Hepatite C Ativação de células iNKT Sangue periférico Dano hepático
Issue Date:	2022
Publisher:	Insight
Citation:	SENFF, Tina et al. Peripheral blood iNKT cell activation correlates with liver damage during acute hepatitis C. JCIinsight, v. 7, n. 2, e155432, p. 1 - 15, Jan. 2022.
DOI:	10.1172/jci. insight.155432.
ISSN:	2379-3708
Copyright:	open access
Appears in Collections:	IOC - Artigos de Periódicos

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