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SARS-COV-2: ULTRASTRUCTURAL CHARACTERIZATION OF MORPHOGENESIS IN AN IN VITRO SYSTEM
Células Vero-E6
Estudos ultraestruturais
Morfogênese
Microscopia eletrônica de transmissão
Microscopia eletrônica de varredura
Modelagem de microscopia eletrônica 3D
Vero-E6 cells
Ultrastructural studies
Morphogenesis
Transmission electron microscopy
Scanning electron microscopy
3D electron microscopy modeling
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Abstract
The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has impacted public health and the world economy and fueled a worldwide race to approve therapeutic and prophylactic agents, but so far there are no specific antiviral drugs. Understanding the biology of the virus is the first step in structuring strategies to combat it, and in this context several studies have been conducted with the aim of understanding the replication mechanism of SARS-CoV-2 in vitro systems. In this work, studies using transmission and scanning electron microscopy and 3D electron microscopy modeling were performed with the goal of characterizing the morphogenesis of SARS-CoV-2 in Vero-E6 cells. Several ultrastructural changes were observed—such as syncytia formation, cytoplasmic membrane projections, lipid droplets accumulation, proliferation of doublemembrane vesicles derived from the rough endoplasmic reticulum, and alteration of mitochondria. The entry of the virus into cells occurred through endocytosis. Viral particles were observed attached to the cell membrane and in various cellular compartments, and extrusion of viral progeny took place by exocytosis. These findings allow us to infer that Vero-E6 cells are highly susceptible to SARS-CoV-2 infection as described in the literature and their replication cycle is similar to that described with SARS-CoV and MERS-CoV in vitro models.
Keywords in Portuguese
SARS-CoV-2.Células Vero-E6
Estudos ultraestruturais
Morfogênese
Microscopia eletrônica de transmissão
Microscopia eletrônica de varredura
Modelagem de microscopia eletrônica 3D
Keywords
SARS-CoV-2.Vero-E6 cells
Ultrastructural studies
Morphogenesis
Transmission electron microscopy
Scanning electron microscopy
3D electron microscopy modeling
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