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BIOLOGICAL CHARACTERIZATION OF YELLOW FEVER VIRUSES ISOLATED FROM NON-HUMAN PRIMATES IN BRAZIL WITH DISTINCT GENOMIC LANDSCAPES
Surtos no Brasil
Marcadores genéticos
Virulência
Neurovirulência do camundongo
Infecção celular
Inibição viral mediada por interferon
Brazil outbreaks
Genetic markers
Virulence
Mouse neurovirulence
Cell infection
Interferon-mediated viral inhibition
Author
Affilliation
Abstract
Since the beginning of the XXI Century, the yellow fever virus (YFV) has been cyclically spreading from the Amazon basin to Brazil’s South and Southeast regions, culminating in an unprecedented outbreak that started in 2016. In this work, we studied four YFV isolated from non-human primates obtained during outbreaks in the states of Rio Grande do Sul in 2008 (PR4408), Goiás (GO05), and Espírito Santo (ES-504) in 2017, and Rio de Janeiro (RJ 155) in 2019. These isolates have genomic differences mainly distributed in non-structural proteins. We compared the isolates’ rates of infection in mammal and mosquito cells and neurovirulence in adult mice. RJ 155 and PR4408 YFV isolates exhibited higher infectivity in mammalian cells and neurovirulence in mice. In mosquito Aag2 cells, GO05 and PR4408 displayed the lowest proliferation rates. These results suggest that RJ 155 and PR4408 YFV isolates carry some genomic markers that increase infectivity in mammal hosts. From this characterization, it is possible to contribute to discovering new molecular markers for the virulence of YFV.
Keywords in Portuguese
Vírus da febre amarelaSurtos no Brasil
Marcadores genéticos
Virulência
Neurovirulência do camundongo
Infecção celular
Inibição viral mediada por interferon
Keywords
Yellow fever virusBrazil outbreaks
Genetic markers
Virulence
Mouse neurovirulence
Cell infection
Interferon-mediated viral inhibition
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