Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/5500
Title: TNF-alpha is expressed at sites of parasite and tissue destruction in the spleen of mice acutely infected with Trypanosoma cruzi.
Other Titles: TNF-alpha is expressed at sites of parasite and tissue destruction in the spleen of mice acutely infected with Trypanosoma cruzi.
Authors: Lima, Elianita Suzart de
Andrade, Zilton de Araújo
Andrade, Sonia Gumes
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil
Abstract: Mice infected with a macrophagotropic strain of Trypanosoma cruzi develop progressive splenomegaly due to reactive hyperplasia with increased number of lymphocytes and macrophages, culminating in parasite disintegration and necrosis of parasitized cells. Necrotic changes have been attributed to the liberation of toxic cytokines, including TNF-alpha, from parasitized macrophages. In the present study, the presence of TNF-alpha was investigated in situ. In addition the participation of destroyed parasites in inducing the liberation of TNF-alpha was examined in two highly susceptible mice strains (C3H and Swiss) and a more resistant strain (DBA). Swiss (90) C3H/He (83) and DBA (30) mice were infected with the Peruvian strain of T. cruzi. Nineteen infected Swiss mice, and 22 infected C3H/He were treated with Benznidazole (one or two doses, 100 mg/kg bw/day), on the 8th and 9th days after infection. Necrotic splenic lesions occurred in both susceptible and resistant strains of mice. Although differing in degree, lesions were more intense in C3H and Swiss than in DBA mice. Comparing untreated and treated susceptible mice, necrotic lesions were significantly less intense in the latter. By specific monoclonal antibody immunolabelling, TNF-alpha was demonstrated in the cytoplasm of macrophages and within necrotic areas, from Swiss, C3H/He and DBA mouse spleens. In conclusion, TNF-alpha, probably synthesized by macrophages, was strongly expressed at the sites of parasite and cell destruction, thus appearing to play a pivotal role in splenic necrotic changes associated with severe experimental T. cruzi infection.
Keywords: Trypanosoma cruzi
Spleen necrosis
Macrophage parasitism
TNF-a
C3H/He mice
DBA mice
Benznidazole
DeCS: Doença de Chagas/metabolismo
Baço/metabolismo
Fator de Necrose Tumoral alfa/metabolismo
Doença Aguda
Doença de Chagas/quimioterapia
Doença de Chagas/patologia
Suscetibilidade a Doenças
Técnicas Imunoenzimáticas
Macrófagos/metabolismo
Camundongos Endogâmicos C3H
Camundongos Endogâmicos DBA
Necrose
Nitroimidazóis/uso terapêutico
Parasitemia/metabolismo
Baço/patologia
Taxa de Sobrevida
Tripanossomicidas/uso terapêutico
Issue Date: 2001
Citation: LIMA, E.S.; ANDRADE, Z.A.; ANDRADE, S.G. TNF-alpha is expressed at sites of parasite and tissue destruction in the spleen of mice acutely infected with Trypanosoma cruzi. International Journal of Experimental Pathology, v. 82, n. 6, p. 327-36, dec. 2001.
Date of defense: 2001
Place of defense: Salvador
Department: Centro de Pesquisas Gonçalo Moniz
Defense institution: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz
ISSN: 0959-9673
Appears in Collections:IGM - Artigos de Periódicos

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