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ENVIRONMENTAL ISOLATION OF SPOROTHRIX BRASILIENSIS IN AN AREA WITH RECURRENT FELINE SPOROTRICHOSIS CASES
Rabello, Vanessa Brito Souza et al. | Date Issued: 2022
Author
Rabello, Vanessa Brito Souza
Almeida-Silva, Fernando
Scramignon-Costa, Bruno de Souza
Motta, Beatriz da Silva
Macedo, Priscila Marques de
Teixeira, Marcus de Melo
Almeida-Paes, Rodrigo
Irinyi, Laszlo
Meyer, Wieland
Zancopé-Oliveira, Rosely Maria
Affilliation
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil.
Universidade de Brasília. Faculdade de Medicina. Núcleo de Medicina Tropical. Brasília, DF, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Marie Bashir Institute for Infectious Diseases and Biosecurity. Westmead Clinical School. Sydney Medical School. Faculty of Medicine and Health. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia / University of Sydney. Westmead Hospital-Research and Education Network. Westmead Institute for Medical Research. Sydney, NSW, Australia.
Marie Bashir Institute for Infectious Diseases and Biosecurity. Westmead Clinical School. Sydney Medical School. Faculty of Medicine and Health. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, NSW, Australia. / University of Sydney. Westmead Hospital-Research and Education Network. Westmead Institute for Medical Research. Sydney, NSW, Australia / Curtin University. Curtin Medical School. Perth, WA, Australia.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Abstract
Sporotrichosis has been expanding throughout the Brazilian territory in recent years. New outbreaks have emerged, and consequently, the sporotrichosis agents, mainly Sporothrix brasiliensis, should remain in the environment somehow. Therefore, the aim of this study was to investigate the presence of Sporothrix spp. in the environment from an area of ​​the Rio de Janeiro state, Brazil, with recurrent cases of human and animal sporotrichosis. Abandoned demolition timber wood samples were collected in the garden of a house where the cases of human and feline sporotrichosis have occurred in the last 10 years. The environmental survey revealed a Sporothrix spp. colony from the serial dilution cultures of one abandoned demolition wood sample. In addition, a fungal strain isolated from a cat with skin lesions that lived in the house was also included in the study. The species-specific PCR, and calmodulin partial sequencing identified the environmental and cat isolates as S. brasiliensis. Furthermore, the phylogenetic analysis performed with the partial sequences of internal transcribed spacer region and constitutive genes (calmodulin, β-tubulin, and chitin synthase) showed high similarity between environmental and cat isolates from the same geographic region. Moreover, the antifungal susceptibility test revealed that the minimal inhibitory concentration of itraconazole from the environment isolate was lower than the cat isolate, while amphotericin B and terbinafine were similar. Our results show that S. brasiliensis is able to maintain itself in the environmental material for years. With this, we corroborate that the eco-epidemiology of sporotrichosis is not well understood, and despite the major occurrence of S. brasiliensis in Brazil, it is rarely isolated from the environment.
Keywords
Brazil
Sporothrix brasiliensis
Environment
Phylogenetic analysis
Sporotrichosis
Timber wood
 
Publisher
Frontiers Media
Citation
RABELLO, Vanessa Brito Souza et al. Environmental Isolation of Sporothrix brasiliensis in an Area With Recurrent Feline Sporotrichosis Cases. Frontiers in cellular and infection microbiology, v. 12, p. 1-7, 2022.
DOI
10.3389/fcimb.2022.894297
ISSN
2235-2988