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BUFFY COAT TRANSCRIPTOMIC ANALYSIS REVEALS ALTERATIONS IN HOST CELL PROTEIN SYNTHESIS AND CELL CYCLE IN SEVERE COVID-19 PATIENTS
SARS-CoV-2
RNA-seq
lncRNA
Biomarcadores
Casaco felpudo
FBL
Pacientes de UTI
Author
Affilliation
Abstract
Transcriptome studies have reported the dysregulation of cell cycle-related genes and
the global inhibition of host mRNA translation in COVID-19 cases. However, the key genes and
cellular mechanisms that are most affected by the severe outcome of this disease remain unclear. For
this work, the RNA-seq approach was used to study the differential expression in buffy coat cells
of two groups of people infected with SARS-CoV-2: (a) Mild, with mild symptoms; and (b) SARS
(Severe Acute Respiratory Syndrome), who were admitted to the intensive care unit with the severe
COVID-19 outcome. Transcriptomic analysis revealed 1009 up-regulated and 501 down-regulated
genes in the SARS group, with 10% of both being composed of long non-coding RNA. Ribosome
and cell cycle pathways were enriched among down-regulated genes. The most connected proteins
among the differentially expressed genes involved transport dysregulation, proteasome degradation,
interferon response, cytokinesis failure, and host translation inhibition. Furthermore, interactome
analysis showed Fibrillarin to be one of the key genes affected by SARS-CoV-2. This protein interacts
directly with the N protein and long non-coding RNAs affecting transcription, translation, and
ribosomal processes. This work reveals a group of dysregulated processes, including translation and
cell cycle, as key pathways altered in severe COVID-19 outcomes.
Keywords in Portuguese
COVID-19SARS-CoV-2
RNA-seq
lncRNA
Biomarcadores
Casaco felpudo
FBL
Pacientes de UTI
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