Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/5625
Title: A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.
Authors: Santos, Ricardo Ribeiro dos
Mengele Junior, José Orivaldo
Postol, Edilberto
Soares, R. A. O
Fernandez, E. Ferreira
Soares, Milena Botelho Pereira
Pontes-de-Carvalho, Lain Carlos
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Imunologia. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Abstract: To study the role of autoreactive T cells in the pathogenesis of cardiomyopathy in Chagas' disease, we generated a cell line by repeated in vitro antigenic stimulation of purified splenic CD4+ T lymphocytes from a chronically Trypanosoma cruzi-infected mouse. Cells from this line were confirmed to be CD4+ CD8- and proliferated upon stimulation with soluble heart antigens from different animal species, as well as with T. cruzi antigen, in the presence of syngeneic feeder cells. In vitro antigen stimulation of the cell line produced a Th1 cytokine profile, with high levels of IFNgamma and IL-2 and absence of IL-4, IL-5 and IL-10. The cell line also terminated the beating of fetal heart clusters in vitro when cocultured with irradiated syngeneic normal spleen cells. In situ injection of the cell line into well established heart transplants also induced the cessation of heart beating. Finally, adoptive transfer of the cell line to heart-immunized or T. cruzi-infected BALB/c nude mice caused intense heart inflammation.
Keywords: T-cell line
Trypanosoma cruzi
Autoreactivity
Carditis
Chagas' disease
DeCS: Linfócitos T CD4-Positivos/imunologia
Doença de Chagas/imunologia
Trypanosoma cruzi
Animais
Linhagem Celular
Doença Crônica
Técnicas de Cocultura
Modelos Animais de Doenças
Rejeição de Enxerto
Transplante de Coração
Interferon gama/análise
Interleucina-2/análise
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Camundongos Endogâmicos DBA
Camundongos Nus
Miocardite/imunologia
Miocárdio/citologia
Miocárdio/imunologia
Coelhos
Ratos
Baço/imunologia
Issue Date: 2001
Citation: SANTOS, R. R. dos et al. A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi. Parasite Immunology, v. 23, n. 2, p. 93-101, feb. 2001.
ISSN: 0141-9838
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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