Author | Andrade, Jó Luis | |
Author | Arruda, Sérgio Marcos | |
Author | Bessa, Theolis Costa Barbosa | |
Author | Paim, Luciana | |
Author | Ramos, Márcio Viana | |
Author | Cavada, Benildo Sousa | |
Author | Barral Netto, Manoel | |
Access date | 2012-10-05T17:40:00Z | |
Available date | 2012-10-05T17:40:00Z | |
Document date | 1999 | |
Citation | ANDRADE, J.L. et al. Lectin-induced nitric oxide production. Cellular Immunology, v. 194, n. 1, p. 98-102, may.1999. | pt_BR |
ISSN | 0008-8749 | |
URI | https://www.arca.fiocruz.br/handle/icict/5650 | |
Language | eng | pt_BR |
Rights | open access | |
Title | Lectin-induced nitric oxide production | pt_BR |
Type | Article | pt_BR |
Abstract | Considering that nitric oxide (NO) may be involved in anti-tumoral and anti-parasite lectin effects, in this report we investigated whether lectin induces NO production. Lectins from Canavalia brasiliensis, Dioclea grandiflora, Pisum arvense (PAA), and concanavalin A induced murine peritoneal cells to produce NO in vitro. PAA induced similar levels to that obtained with lipopolysaccharide plus interferon-gamma. NO production by adherent cells was significantly lower than that of unfractionated cells, suggesting a combination of lectin stimuli directly on macrophages and via lymphocyte stimulation. Ex vivo experiments showed that cells stimulated in vivo could maintain NO production in vitro without further stimuli. NO synthesis blockage in vivo can significantly increase cell numbers in draining lymph nodes after lectin injection compared to unblocked controls, suggesting an in vivo association of lectin stimuli and NO production. Taken together these data show that lectins can induce NO production both in vitro and in vivo. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal do Ceará. Departamento de Bioquímica e Biologia Molecular. Fortaleza, CE, Brasil | pt_BR |
Affilliation | Universidade Federal do Ceará. Departamento de Bioquímica e Biologia Molecular. Fortaleza, CE, Brasil | pt_BR |
Affilliation | Universidade Federal do Ceará. Departamento de Bioquímica e Biologia Molecular. Fortaleza, CE, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Subject | Nitric Oxide | pt_BR |
Subject | Adherent cells | pt_BR |
Subject | Peritoneal cells | pt_BR |
Subject | Macrophages | pt_BR |
Subject | Immunology | pt_BR |
Subject | Canavalia Brasiliensis | pt_BR |
Subject | Canavalia Ensiformis | pt_BR |
Subject | Concanavalin A | pt_BR |
Subject | ConA | pt_BR |
Subject | Dioclea Grandiflora | pt_BR |
Subject | Pisum Arvense | pt_BR |
DeCS | Lectinas/imunologia | pt_BR |
DeCS | Óxido Nítrico/biossíntese | pt_BR |
DeCS | Lectinas de Plantas | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Células Cultivadas | pt_BR |
DeCS | Concanavalina A/imunologia | pt_BR |
DeCS | Concanavalina A/farmacologia | pt_BR |
DeCS | Feminino | pt_BR |
DeCS | Lectinas/farmacologia | pt_BR |
DeCS | Camundongos | pt_BR |
DeCS | Camundongos Endogâmicos BALB C | pt_BR |
DeCS | Peritônio/citologia | pt_BR |