Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/5650
Title: Lectin-induced nitric oxide production
Authors: Andrade, Jó Luis
Arruda, Sérgio Marcos
Bessa, Theolis Costa Barbosa
Paim, Luciana
Ramos, Márcio Viana
Cavada, Benildo Sousa
Barral Netto, Manoel
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal do Ceará. Departamento de Bioquímica e Biologia Molecular. Fortaleza, CE, Brasil
Universidade Federal do Ceará. Departamento de Bioquímica e Biologia Molecular. Fortaleza, CE, Brasil
Universidade Federal do Ceará. Departamento de Bioquímica e Biologia Molecular. Fortaleza, CE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Abstract: Considering that nitric oxide (NO) may be involved in anti-tumoral and anti-parasite lectin effects, in this report we investigated whether lectin induces NO production. Lectins from Canavalia brasiliensis, Dioclea grandiflora, Pisum arvense (PAA), and concanavalin A induced murine peritoneal cells to produce NO in vitro. PAA induced similar levels to that obtained with lipopolysaccharide plus interferon-gamma. NO production by adherent cells was significantly lower than that of unfractionated cells, suggesting a combination of lectin stimuli directly on macrophages and via lymphocyte stimulation. Ex vivo experiments showed that cells stimulated in vivo could maintain NO production in vitro without further stimuli. NO synthesis blockage in vivo can significantly increase cell numbers in draining lymph nodes after lectin injection compared to unblocked controls, suggesting an in vivo association of lectin stimuli and NO production. Taken together these data show that lectins can induce NO production both in vitro and in vivo.
Keywords: Nitric Oxide
Adherent cells
Peritoneal cells
Macrophages
Immunology
Canavalia Brasiliensis
Canavalia Ensiformis
Concanavalin A
ConA
Dioclea Grandiflora
Pisum Arvense
DeCS: Lectinas/imunologia
Óxido Nítrico/biossíntese
Lectinas de Plantas
Animais
Células Cultivadas
Concanavalina A/imunologia
Concanavalina A/farmacologia
Feminino
Lectinas/farmacologia
Camundongos
Camundongos Endogâmicos BALB C
Peritônio/citologia
Issue Date: 1999
Citation: ANDRADE, J.L. et al. Lectin-induced nitric oxide production. Cellular Immunology, v. 194, n. 1, p. 98-102, may.1999.
ISSN: 0008-8749
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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