Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/5706
Title: Evaluation of spleen cell population and effect of splenectomy on granuloma modulation in BALB/c mice infected with Schistosoma mansoni.
Authors: Arruda, Sérgio Marcos
Santoro, Ferrucio
Sadigursky, Mauricio
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Institut Pasteur. Centro d'Immunologie et de Biologie Parasitaire. Lille, França
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Abstract: A kinetic study of the cells present in the spleen of BALB/c mice infected with Schistosoma mansoni was carried out. The lymphocytes were evaluated phenotypically with monoclonal antibodies and the effect of splenectomy on the modulation of periovular granuloma was also investigated. The infected mice had proportional increases in the numbers of neutrophils, plasma cells, macrophages and eosinophils in the spleen. The largest number of neutrophil, plasma cells and macrophage were found between the 8th and the 12th week of infection, while the amount of eosinophils were higher later on, around the 20th week. The lymphocytes phenotypically characterized as Thy 1.2, Lyt 1.2 (CD4) increased mildly in proportional numbers. However, the percentage of lymphocytes with the Lyt 2.2 (CD8) phenotype, which is characteristic of suppressor and cytotoxic T cells, increased significantly with the progress of the disease. The numbers of B lymphocytes, which comprise 50% of the mononuclear cells present in the spleen, increased significantly till the 16th week when they began to decrease. The mean diameters of periovular granulomas were comparatively similar in both experimental groups (splenectomized and non-splenectomized mice). However, the evolutive types of granuloma (exudative, intermediate and fibrous) in splenectomized mice were proportionally different from those of non splenectomized mice in the 16th and 24th week of infection. It is inferred that lymphnodes or other secondary lymphoid organs, in the absence of the spleen, assume a modulating action on periovular granulomas, although the evolution of the granulomas is somehow delayed in splenectomized mice.
Keywords: Schistosoma mansoni
Murine schistosomiasis
Granuloma modulation
Splenectomy
Lymphocytes
DeCS: Esquistossomose mansoni/patologia
Baço/patologia
Esplenectomia
Animais
Granuloma/patologia
Hepatopatias/patologia
Camundongos
Camundongos Endogâmicos BALB C
Tamanho do Órgão
Issue Date: 1993
Citation: ARRUDA, S.M.; SANTORO, F.; SADIGURSKY, M. Evaluation of spleen cell population and effect of splenectomy on granuloma modulation in BALB/c mice infected with Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, v. 88, n. 1, p. 97-102, jan.-mar. 1993.
ISSN: 0074-0276
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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