Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/5722
Title: Association of elevated anti-sarcolemma, anti-idiotype antibody levels with the clinical and pathologic expression of chronic Chagas myocarditis
Authors: Sadigursky, Moysés
von Kreuter, Betsy F.
Ling, Pei-Ying
Buch, Charles A. Santos
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Cornell University Medical College. NY, New York.
Cornell University Medical College. NY, New York.
Cornell University Medical College. NY, New York.
Abstract: Antibody F(ab')2 fragments derived from the sera of four patients with histology-proven chronic Chagas myocarditis [cF(ab')2]-complexed antibody F(ab')2 fragments of children with acute Trypanosoma cruzi infection [aF(ab')2] in significantly higher molar ratios than those measured with F(ab')2 antibody fragments of normal subjects [nF(ab')2] from nonendemic areas (p less than 0.05). Anti-idiotype [cF(ab')2 X aF(ab')2] immune-complex formation was significantly blunted by preabsorption of cF(ab')2 with porcine heart atria sarcolemma (PAMs) immobilized on sepharose beads (inhibition, mean, 78.1 +/- 2.4%, n = 4). cF(ab')2 X nF(ab')2] immune-complex formation was also inhibited by pretreatment of cF(ab')2 with PAMs (inhibition, mean, 48.7 +/- 7.5%, n = 4). The sera of three groups of subjects from a geographic zone endemic for T. cruzi infection in the northeast of Brazil were assayed for free and immune-complexed IgG anti-acute T. cruzi infection F(ab')2. The indexed levels of free IgG anti-idiotype antibody activity and levels of IgG anti-idiotype immune complexes (IC') were markedly elevated in hospitalized patients with severe, decompensated chronic Chagas myocarditis (n = 23), and their IC' indexes were significantly higher than those measured in asymptomatic seropositive subjects from a nearby endemic village of the northeast of Brazil (Moniz Ferreira, n = 92, p less than 0.001) and in healthy seronegative villagers (n = 84, p less than 0.001). There exists a strong correlation between elevated IgG anti-sarcolemma, anti-idiotype activity levels and the clinical and pathologic expression of chronic Chagas myocarditis.
DeCS: Anticorpos Antiidiotípicos/imunologia
Anticorpos Antiprotozoários/imunologia
Cardiomiopatia Chagásica/imunologia
Fragmentos Fab das Imunoglobulinas/imunologia
Imunoglobulina G/imunologia
Miocardite/imunologia
Adulto
Animais
Complexo Antígeno-Anticorpo/imunologia
Criança
Humanos
Miocardite/etiologia
Sarcolema/imunologia
Trypanosoma cruzi/imunologia
Issue Date: 1989
Citation: SADIGURSKY, Moysés et al. Association of elevated anti-sarcolemma, anti-idiotype antibody levels with the clinical and pathologic expression of chronic Chagas myocarditis. Circulation, v. 80, n. 5, p. 1269-1276, Nov. 1989.
ISSN: 0009-7322
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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