| Author | Guerra- Slompo, Eloise Pavão | |
| Author | Picchi-Constante, Gisele Fernanda Assine | |
| Author | Marek, Martin | |
| Author | Romier, Christophe | |
| Author | Sippl, Wolfgang | |
| Author | Zanchin, Nilson Ivo Tonin | |
| Access date | 2023-03-15T18:05:19Z | |
| Available date | 2023-03-15T18:05:19Z | |
| Document date | 2023 | |
| Citation | GUERRA-SLOMPO, Eloise Pavão et al. In cellulo and in vivo assays for compound testing against Trypanosoma cruzi. STAR Protocols, v. 4, n. 102058, p. 1–42, 2023. | en_US |
| ISSN | 2666-1667 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/57387 | |
| Language | por | en_US |
| Publisher | Cell Press | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Trypanosoma cruzi | en_US |
| Subject in Portuguese | Ensaios celulares | en_US |
| Subject in Portuguese | Ensaios in vivo | en_US |
| Subject in Portuguese | Benznidazole | en_US |
| Title | In cellulo and in vivo assays for compound testing against Trypanosoma cruzi | en_US |
| Type | Article | en_US |
| DOI | 10.1016/j.xpro.2023.102058 | |
| Abstract | Here, we describe a combined in cellulo and in vivo approach to identify compounds with higher potential for efficient inhibition of Trypanosoma cruzi. Phase I of in cellulo assays is designed to exclude inactive or toxic compounds, while phase II is designed for accurate IC50, CC50, and selective index (SI) determination. Compounds showing high SI are tested using in vivo infection models in parallel with benznidazole to assess their efficacy relative to a reference drug used for Chagas disease treatment. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Université de Strasbourg, CNRS, INSERM. Institut de Génétique et de Biologie Moléculaire et Cellulaire. Illkirch, France. / Université de Strasbourg, CNRS, INSERM. Institut de Génétique et de Biologie Moléculaire et Cellulaire. Department of Integrated Structural Biology. Illkirch, France. / Loschmidt Laboratories. Department of Experimental Biology & RECETOX. Faculty of Science. Masaryk University, Czech Republic. | en_US |
| Affilliation | Université de Strasbourg, CNRS, INSERM. Institut de Génétique et de Biologie Moléculaire et Cellulaire. Illkirch, France. / Université de Strasbourg, CNRS, INSERM. Institut de Génétique et de Biologie Moléculaire et Cellulaire. Department of Integrated Structural Biology. Illkirch, France. | en_US |
| Affilliation | Institute of Pharmacy. Martin-Luther-Universität Halle-Wittenberg. Halle (Saale), Germany. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Subject | Chagas Disease | en_US |
| Subject in Spanish | Enfermedad de Chagas | en_US |
| Subject in French | Maladie de Chagas | en_US |
| DeCS | Doença de Chagas | en_US |
