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IN CELLULO AND IN VIVO ASSAYS FOR COMPOUND TESTING AGAINST TRYPANOSOMA CRUZI
Guerra- Slompo, Eloise Pavão et al. | Date Issued: 2023
Author
Guerra- Slompo, Eloise Pavão
Picchi-Constante, Gisele Fernanda Assine
Marek, Martin
Romier, Christophe
Sippl, Wolfgang
Zanchin, Nilson Ivo Tonin
Affilliation
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Université de Strasbourg, CNRS, INSERM. Institut de Génétique et de Biologie Moléculaire et Cellulaire. Illkirch, France. / Université de Strasbourg, CNRS, INSERM. Institut de Génétique et de Biologie Moléculaire et Cellulaire. Department of Integrated Structural Biology. Illkirch, France. / Loschmidt Laboratories. Department of Experimental Biology & RECETOX. Faculty of Science. Masaryk University, Czech Republic.
Université de Strasbourg, CNRS, INSERM. Institut de Génétique et de Biologie Moléculaire et Cellulaire. Illkirch, France. / Université de Strasbourg, CNRS, INSERM. Institut de Génétique et de Biologie Moléculaire et Cellulaire. Department of Integrated Structural Biology. Illkirch, France.
Institute of Pharmacy. Martin-Luther-Universität Halle-Wittenberg. Halle (Saale), Germany.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Abstract
Here, we describe a combined in cellulo and in vivo approach to identify compounds with higher potential for efficient inhibition of Trypanosoma cruzi. Phase I of in cellulo assays is designed to exclude inactive or toxic compounds, while phase II is designed for accurate IC50, CC50, and selective index (SI) determination. Compounds showing high SI are tested using in vivo infection models in parallel with benznidazole to assess their efficacy relative to a reference drug used for Chagas disease treatment.
Keywords in Portuguese
Trypanosoma cruzi
Ensaios celulares
Ensaios in vivo
Benznidazole
 
Keywords
Chagas Disease
Keywords in Spanish
Enfermedad de Chagas
DeCS
Doença de Chagas
Publisher
Cell Press
Citation
GUERRA-SLOMPO, Eloise Pavão et al. In cellulo and in vivo assays for compound testing against Trypanosoma cruzi. STAR Protocols, v. 4, n. 102058, p. 1–42, 2023.
DOI
10.1016/j.xpro.2023.102058
ISSN
2666-1667