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ESTABLISHMENT OF A MURINE MODEL OF CONGENITAL TOXOPLASMOSIS AND VALIDATION OF A QPCR ASSAY TO ASSESS THE PARASITE LOAD IN MATERNAL AND FETAL TISSUES
Toxoplasmose congênita
Transmissão vertical
Modelo murino endogâmico
Webster suíço
Carga parasita
PCR quantitativo em tempo real
Congenital toxoplasmosis
Vertical transmission
Outbred murine model
Swiss Webster
Parasite load
Quantitative real-time PCR
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Plataforma de PCR em Tempo Real RPT09A. Rio de Janeiro, RJ, Brasil / The University of Texas at El Paso. Department of Biological Science, Border Biomedical Research Center. El Paso, TX, United States.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Plataforma de PCR em Tempo Real RPT09A. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Plataforma de PCR em Tempo Real RPT09A. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Plataforma de PCR em Tempo Real RPT09A. Rio de Janeiro, RJ, Brasil / The University of Texas at El Paso. Department of Biological Science, Border Biomedical Research Center. El Paso, TX, United States.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Plataforma de PCR em Tempo Real RPT09A. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Plataforma de PCR em Tempo Real RPT09A. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.
Abstract
Toxoplasma gondii is the causative agent of toxoplasmosis, a disease that affects
warm-blooded animals and one third of the human population worldwide.
Pregnant women who have never been exposed to the parasite constitute an
important risk group, as infection during pregnancy often leads to congenital
toxoplasmosis, the most severe form of the disease. Current therapy for
toxoplasmosis is the same as it was 50 years ago and has little or no effect when
vertical transmission occurs. Therefore, it is urgent to develop new strategies
to prevent mother-to-fetus transmission. The implementation of experimental
animal models of congenital toxoplasmosis that reproduces the transmission
rates and clinical signs in humans opens an avenue of possibilities to interfere
in the progression of the disease. In addition, knowing the parasite load in
maternal and fetal tissues after infection, which may be related to organ
abnormalities and disease outcome, is another important step in designing
a promising intervention strategy. Therefore, we implemented here a murine
model of congenital toxoplasmosis with outbred Swiss Webster mice infected
intravenously with tachyzoites of the ME49 strain of T. gondii that mimics the
frequency of transmission of the parasite, as well as important clinical signs of
human congenital toxoplasmosis, such as macrocephaly, in addition to providing
a highly sensitive quantitative real-time PCR assay to assess parasite load in mouse
tissues. As the disease is not restricted to humans, also affecting several domestic
animals, including companion animals and livestock, they can also benefit from
the model presented in this study.
Keywords in Portuguese
Toxoplasma gondiiToxoplasmose congênita
Transmissão vertical
Modelo murino endogâmico
Webster suíço
Carga parasita
PCR quantitativo em tempo real
Keywords
Toxoplasma gondiiCongenital toxoplasmosis
Vertical transmission
Outbred murine model
Swiss Webster
Parasite load
Quantitative real-time PCR
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