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https://www.arca.fiocruz.br/handle/icict/57942
RED MEAT INTAKE, INDOLE-3-ACETATE, AND DOREA LONGICATENA TOGETHER AFFECT INSULIN RESISTANCE AFTER GASTRIC BYPASS
Roux-en-Y Gastric bypass
food intake
indole-3-acetate
insulin resistance
metabolomics
red meat
tryptophan metabolism
type 2 diabetes
Author
Affilliation
Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. Department of Gastroenterology. Laboratory of Nutrition and Metabolic Surgery of the Digestive System. São Paulo, SP, Brazil.
Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. Department of Gastroenterology. Laboratory of Nutrition and Metabolic Surgery of the Digestive System. São Paulo, SP, Brazil.
Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. Department of Gastroenterology. Laboratory of Nutrition and Metabolic Surgery of the Digestive System. São Paulo, SP, Brazil.
Hospital Sírio Libanês. Department of Medical Clinical Nutrition. Brasilia, DF, Brazil.
Faculty of Nutrition. Universidade São Camilo. São Paulo, SP, Brazil.
Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brazil.
Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. Department of Gastroenterology. Laboratory of Nutrition and Metabolic Surgery of the Digestive System. São Paulo, SP, Brazil.
Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. Department of Gastroenterology. Laboratory of Nutrition and Metabolic Surgery of the Digestive System. São Paulo, SP, Brazil.
Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. Department of Gastroenterology. Laboratory of Nutrition and Metabolic Surgery of the Digestive System. São Paulo, SP, Brazil.
Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. Department of Gastroenterology. Laboratory of Nutrition and Metabolic Surgery of the Digestive System. São Paulo, SP, Brazil.
Hospital Sírio Libanês. Department of Medical Clinical Nutrition. Brasilia, DF, Brazil.
Faculty of Nutrition. Universidade São Camilo. São Paulo, SP, Brazil.
Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brazil.
Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. Department of Gastroenterology. Laboratory of Nutrition and Metabolic Surgery of the Digestive System. São Paulo, SP, Brazil.
Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. Department of Gastroenterology. Laboratory of Nutrition and Metabolic Surgery of the Digestive System. São Paulo, SP, Brazil.
Abstract
Roux-en-Y Gastric bypass (RYGB) promotes improvement in type 2 diabetes (T2D) shortly after surgery, with metabolic mechanisms yet to be elucidated. This study aimed to investigate the relationship between food intake, tryptophan metabolism, and gut microbiota on the glycemic control of obese T2D women after RYGB surgery. Twenty T2D women who underwent RYGB were evaluated before and three months after surgery. Food intake data were obtained by a seven-day food record and a food frequency questionnaire. Tryptophan metabolites were determined by untargeted metabolomic analysis, and the gut microbiota was determined by 16S rRNA sequencing. The glycemic outcomes were fasting blood glucose, HbA1C, HOMA-IR, and HOMA-beta. Linear regression models were applied to assess the associations between the changes in food intake, tryptophan metabolism, and gut microbiota on glycemic control after RYGB. All variables changed after RYGB (p < 0.05), except for tryptophan intake. Jointly, the variation in red meat intake, plasma indole-3-acetate, and Dorea longicatena was associated with postoperative HOMA-IR {R2 0.80, R2 adj 0.74; p < 0.01}. Red meat intake decreased three months after bariatric surgery while indole-3-acetate and Dorea longicatena increased in the same period. These combined variables were associated with better insulin resistance in T2D women after RYGB.
Keywords
Gut microbiotaRoux-en-Y Gastric bypass
food intake
indole-3-acetate
insulin resistance
metabolomics
red meat
tryptophan metabolism
type 2 diabetes
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