Author
Affilliation
Abstract
SARS-CoV-2 (SCoV2) and its variants of concern pose
serious challenges to the public health. The variants increased
challenges to vaccines, thus necessitating for development of new
intervention strategies including anti-virals. Within the international
Covid19-NMR consortium, we have identified binders targeting the
RNA genome of SCoV2. We established protocols for the production
and NMR characterization of more than 80% of all SCoV2 proteins.
Here, we performed an NMR screening using a fragment library for
binding to 25 SCoV2 proteins and identified hits also against
previously unexplored SCoV2 proteins. Computational mapping was
used to predict binding sites and identify functional moieties
(chemotypes) of the ligands occupying these pockets. Striking
consensus was observed between NMR-detected binding sites of
the main protease and the computational procedure. Our
investigation provides novel structural and chemical space for
structure-based drug design against the SCoV2 proteome.
Publisher
Wiley-VCH
Citation
BERG, Hannes et al. Comprehensive Fragment Screening of the SARS-CoV-2 Proteome Explores Novel Chemical Space for Drug Development. Angew. Chem. Int. Ed., 2022, e202205858.
DOI
10.1002/anie.202205858
ISSN
1521-3773
Notes
Francisco Gomes Neto - Fundação 0swaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Demais Autores:
Hannes Berg,[a,b‡] Maria A. Wirtz Martin,[a,b‡] Nadide Altincekic,[a,b‡] Islam Alshamleh,[a,b] Jasleen Kaur Bains,[a,b] Julius
Blechar,[a] Betül Ceylan,[a,b] Vanessa de Jesus,[a,b] Karthikeyan Dhamotharan,[b,c] Christin Fuks,[a] Santosh L. Gande,[a,b] Bruno
Hargittay,[a,b] Katharina F. Hohmann,[a,b] Marie T. Hutchison,[a,b] Sophie Marianne Korn,[b,c] Robin Krishnathas,[a,b] Felicitas
Kutz,[a,b] Verena Linhard,[a,b] Tobias Matzel,[a,b] Nathalie Meiser,[a] Anna Niesteruk,[a,b] Dennis J. Pyper,[a,b] Linda Schulte,[a,b]
Sven Trucks,[a] Kamal Azzaoui,[d] Marcel J. J. Blommers,[d] Yojana Gadiya,[e,f] Reagon Karki,[e,f] Andrea Zaliani,[e,f] Philip
Gribbon,[e,f] Marcius da Silva Almeida,[g,h] Cristiane Dinis Anobom,[i,j] Anna L. Bula,[k] Matthias Bütikofer,[l] Ícaro Putinhon
Caruso,[g,m] Isabella Caterina Felli,[n,o] Andrea T. Da Poian,[g] Gisele Cardoso de Amorim,[g,p] Nikolaos K. Fourkiotis,[q] Angelo
Gallo,[q,r] Dhiman Ghosh, i,s] Oksana Gorbatyuk,[t] Bing Hao,[t] Vilius Kurauskas,[u] Lauriane Lecoq,[v]
Yunfeng Li,[t] Nathane Cunha Mebus-Antunes,[g] Miguel Mompeán,[w] Thais Cristtina Neves-Martins,[g] Martí Ninot-Pedrosa,[v]
Anderson S. Pinheiro,[j] Letizia Pontoriero,[n,o] Yulia Pustovalova,[t] Roland Riek,[l] Angus J. Robertson,[x] Marie Jose Abi
Saad,[y] Miguel Á. Treviño,[w] Aikaterini C. Tsika,[q] Fabio C. L. Almeida,[g,i] Ad Bax,[x] Katherine Henzler-Wildman,[u] Jeffrey C.
Hoch,[t] Kristaps Jaudzems,[k] Douglas V. Laurents,[w] Julien Orts,[y] Roberta Pierattelli,[n,o] Georgios A. Spyroulias,[q] Elke
Duchardt-Ferner,[b,c] Jan Ferner,[a,b] Boris Fürtig,[a,b] Martin Hengesbach,[a] Frank Löhr,[b,c] Nusrat Qureshi,[a,b] Christian
Richter,[a,b] Krishna Saxena,[a,b] Andreas Schlundt,[b,c] Sridhar Sreeramulu,[a,b] Anna Wacker,[a,b] Julia E. Weigand,[z] Julia
Wirmer-Bartoschek,[a,b] Jens Wöhnert,[b,c] and Harald Schwalbe*[a,b].
Notas- Instituições:
[a,b] H. Berg[‡], M. A. Wirtz Martin[‡], N. Altincekic[‡], Dr. I. Alshamleh, J. K. Bains, J. Blechar, B. Ceylan, V. de Jesus, C. Fuks, Dr. S. L. Gande, B. Hargittay, K. F.
Hohmann, M. T. Hutchison, R. Krishnathas, F. Kutz, V. Linhard, T. Matzel, N. Meiser, Dr. A. Niesteruk, D. J. Pyper, Dr. L. Schulte, Dr. S. Trucks, Dr. J.
Ferner, Dr. B. Fürtig, Dr. M. Hengesbach, Dr. N. Qureshi, Dr. C. Richter, Dr. K. Saxena, Dr. S. Sreeramulu, Dr. A. Wacker, Dr. J. Wirmer-Bartoschek, Dr.
Prof. Dr. H. Schwalbe
[a] Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany
[b] Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany
Max-von-LaueStrasse 7+9, 60438 Frankfurt am Main (Germany)
E-mail: schwalbe@nmr.uni-frankfurt.de
[b,c] K. Dhamotharan, Dr. S. M. Korn, Dr. E. Duchardt-Ferner, Dr. F. Löhr, Dr. A. Schlundt, Prof. Dr. J. Wöhnert
[c] Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany
Max-von-Laue Strasse 7+9, 60438 Frankfurt am Main (Germany)
[d] Dr. K. Azzaoui, Dr. M. J. J. Blommers
Saverna Therapeutics, Pumpmattenweg 3, 4105 Biel-Benken, Switzerland
[e,f] Y. Gadiya, R. Karki, A. Zaliani, Dr. P. Gribbon
[e] Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP), Screening Port, Schnackenburgallee 114, 22525 Hamburg, Germany
[f] Fraunhofer Cluster of Excellence for Immune-Mediated Diseases (CIMD), Theodor-Stern-Kai 7, 60596 Frankfurt am Main, Germany
[g,h] M. S. Almeida, A. T. Da Poian, N. C. Mebus-Antunes, T. C. Neves-Martins, F. C. L. Almeida
[g] Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
[h] Fraunhofer Cluster of Excellence for Immune-Mediated Diseases (CIMD), Theodor-Stern-Kai 7, 60596 Frankfurt am Main, Germany
[i,j] C. D Anobom, A. S. Pinheiro
[i] National Center of Nuclear Magnetic Resonance (CNRMN), CENABIO, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
[j] Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
[k] A. L. Bula, K. Jaudzems
[k] Latvian Institute of Organic Synthesis, Aizkraukles 21, LV-1006 Riga, Latvia
[l] M. Bütikofer, D. Ghosh, R. Riek
[l] ETH, Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, HCI F217, Vladimir-Prelog-Weg 2, 8093 Zürich, Switzerland.
[g,m] Í. P. Caruso
[m] Multiuser Center for Biomolecular Innovation (CMIB), Department of Physics, São Paulo State University (UNESP), São José do Rio Preto, Brazil
[n,o] I. C. Felli, L. Pontoriero, R. Pierattelli
[n] Magnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, Sesto Fiorentino, 50019, Florence, Italy
[o] Department of Chemistry "Ugo Schiff", University of Florence, Via della Lastruccia 3-13, Sesto Fiorentino, 50019, Florence, Italy
[g,p] G. C. de Amorim
[p] Multidisciplinary Center for Research in Biology (NUMPEX), Campus Duque de Caxias Federal University of Rio de Janeiro, Duque de Caxias, Brazil
[q] N. K. Fourkiotis, A. C. Tsika, G. A. Spyroulias
[q] Department of Pharmacy, University of Patras, Patras, Greece
[q,r] A. Gallo,
[r] Department of Chemistry, University of Torino IT -10126 Torino. Italy
[i,s] F. Gomes-Neto
[s] Laboratory of Toxinology, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil
[t] O. Gorbatyuk, B. Hao, Y. Li, Y. Pustovalova, J. C. Hoch
[t] Department of Molecular Biology and Biophysics UConn Health 263 Farmington Ave., Farmington, CT 06030-3305 USA
[u] V. Kurauskas, K. Henzler-Wildman
[u] Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706
[v] L. Lecoq, M. Ninot-Pedrosa
[v] Molecular Microbiology and Structural Biochemistry, UMR5086 CNRS/Université Lyon 1, 7, passage du Vercors, 69367 Lyon, France.
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