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2025
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MYCOBACTERIUM TUBERCULOSIS INDUCES DELAYED LIPID DROPLET ACCUMULATION IN DENDRITIC CELLS DEPENDING ON BACTERIAL VIABILITY AND VIRULENCE
Costa, Maria Fernanda de Souza et al. | Date Issued: 2022
Author
Costa, Maria Fernanda de Souza
Dutra, Filipe Pereira
Deboosere, Nathalie
Jouny, Samuel
Ryul Song, Ok-
Iack, Guilherme
Souza, Andreia Lamoglia
Roma, Eric Henrique
Delorme, Vincent
Bozza, Patrícia T.
Brodin, Priscille
Affilliation
Universidade Federal Fluminense. Instituto de Biologia. Departamento de Imunobiologia. Niterói, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento tecnológico em Saúde. Rio de janeiro, RJ, Brasil / Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 – UMR 9017 - CIIL - Center for Infection and Immunity of Lille, Lille, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.
Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 – UMR 9017 - CIIL - Center for Infection and Immunity of Lille, Lille, France.
Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 – UMR 9017 - CIIL - Center for Infection and Immunity of Lille, Lille, France.
Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 – UMR 9017 - CIIL - Center for Infection and Immunity of Lille, Lille, France.
Universidade Federal Fluminense. Instituto de Biologia. Departamento de Imunobiologia. Niterói, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil..
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Imunologia e Imunogenética em Doenças Infecciosas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Imunologia e Imunogenética em Doenças Infecciosas. Rio de Janeiro, RJ, Brasil.
Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 – UMR 9017 - CIIL - Center for Infection and Immunity of Lille, Lille, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.
Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 – UMR 9017 - CIIL - Center for Infection and Immunity of Lille, Lille, France.
Abstract
Tuberculosis remains a global health threat with high morbidity. Dendritic cells (DCs) participate in the acute and chronic inflammatory responses to Mycobacterium tuberculosis (Mtb) by directing the adaptive immune response and are present in lung granulomas. In macrophages, the interaction of lipid droplets (LDs) with mycobacteriacontaining phagosomes is central to host-pathogen interactions. However, the data available for DCs are still a matter of debate. Here, we reported that bone marrowderived DCs (BMDCs) were susceptible to Mtb infection and replication at similar rate to macrophages. Unlike macrophages, the analysis of gene expression showed that Mtb infection induced a delayed increase in lipid droplet-related genes and proinflammatory response. Hence, LD accumulation has been observed by high-content imaging in late periods. Infection of BMDCs with killed H37Rv demonstrated that LD accumulation depends on Mtb viability. Moreover, infection with the attenuated strains H37Ra and Mycobacterium bovis-BCG induced only an early transient increase in LDs, whereas virulent Mtb also induced delayed LD accumulation. In addition, infection with the BCG strain with the reintroduced virulence RD1 locus induced higher LD accumulation and bacterial replication when compared to parental BCG. Collectively, our data suggest that delayed LD accumulation in DCs is dependent on mycobacterial viability and virulence.
Keywords in Portuguese
Mycobacterium tuberculosis
induz gota lipídica retardada acúmulo
Células dendríticas
Dependendo da viabilidade bacteriana e virulência
 
Keywords
Mycobacterium tuberculosis
Induces delayed lipid droplet accumulation
Dendritic cells depending
Bacterial viability and virulence
 
Publisher
Wiley
Citation
COSTA, Maria Fernanda de Souza et al. Mycobacterium tuberculosis induces delayed lipid droplet accumulation in dendritic cells depending on bacterial viability and virulence. Wiley,119, p.224-236, Dec. 2022.
DOI
10.1111/mmi.15023
ISSN
1365-2958